We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.
REFERENCES
1.
M.
Arifuzzaman
, M.
Maeda
, A.
Itoh
, K.
Nishikata
, C.
Takita
, R.
Saito
, T.
Ara
, K.
Nakahigashi
, H. C.
Huang
, A.
Hirai
, K.
Tsuzuki
, S.
Nakamura
, M.
Altaf-Ul-Amin
, T.
Oshima
, T.
Baba
, N.
Yamamoto
, T.
Kawamura
, T.
Ioka-Nakamichi
, M.
Kitagawa
, M.
Tomita
, S.
Kanaya
, C.
Wada
, and H.
Mori
, Genome Res.
16
, 686
(2006
).2.
J. R.
Parrish
, K. D.
Gulyas
, and R. L.
Finley
, Jr., Curr. Opin. Biotechnol.
17
, 387
(2006
).3.
R. B.
Jones
, A.
Gordus
, J. A.
Krall
, and G.
MacBeath
, Nature (London)
439
, 168
(2006
).4.
S. J.
Maerkl
and S. R.
Quake
, Science
315
, 233
(2007
).5.
T. A.
Morton
and D. G.
Myszka
, Methods Enzymol.
295
, 268
(1998
).6.
C. T.
Campbell
and G.
Kim
, Biomaterials
28
, 2380
(2007
).7.
R.
Karlsson
, J. Mol. Recognit.
17
, 151
(2004
).8.
K.
Andersson
, D.
Areskoug
, and E.
Hardenborg
, J. Mol. Recognit.
12
, 310
(1999
).9.
Y.
Luo
, F.
Yu
, and R. N.
Zare
, Lab Chip
8
, 694
(2008
).10.
D.
Gerber
, S. J.
Maerkl
, and S. R.
Quake
, Nat. Methods
6
, 71
(2009
).© 2009 American Institute of Physics.
2009
American Institute of Physics
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