We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.
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Research Article| August 21 2009
Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device
Steven R. Bates;
Stephen R. Quake
Steven R. Bates, Stephen R. Quake; Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device. Appl. Phys. Lett. 17 August 2009; 95 (7): 073705. https://doi.org/10.1063/1.3211382
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