Cerium oxide-chitosan based nanobiocomposite for food borne mycotoxin detection

Cerium oxide nanoparticles $(NanoCeO2)$ and chitosan (CH) based nanobiocomposite film deposited onto indium-tin-oxide coated glass substrate has been used to coimmobilize rabbit immunoglobin (r-IgGs) and bovine serum albumin (BSA) for food borne mycotoxin [ochratoxin-A (OTA)] detection. Electrochemical studies reveal that presence of $NanoCeO2$ increases effective electro-active surface area of $CH-NanoCeO2/$indium tin oxide (ITO) nanobiocomposite resulting in high loading of r-IgGs. $BSA/r-IgGs/CH-NanoCeO2/ITO$ immunoelectrode exhibits improved linearity (0.25–6.0 ng/dl), detection limit (0.25 ng/dl), response time (25 s), sensitivity $(18μA/ngdl−1cm−2)$⁠, and regression coefficient $(r2∼0.997)$⁠.