Comprehensive analysis of fluorescence of albumin shows a weak fluorescence band at 430 nm, whose intensity exhibits a remarkable sensitivity to the presence of heavy ions in water. Using this fluorescence as a marker, as low as 10 pM concentration of lead can be routinely detected. Such a great sensitivity is explained in terms of electrostatic interactions in solution, which promote protein agglomeration. The latter is independently confirmed using dynamic light scattering measurements.

1.
H.
Needleman
,
Annu. Rev. Med.
55
,
209
(
2004
).
2.
J.
Markovac
and
G. W.
Goldstein
,
Nature (London)
334
,
71
(
1988
).
3.
E.
Rossi
,
Clin. Biochem.
29
,
63
(
2008
).
4.
J.
Schwartz
,
C.
Angle
, and
H.
Pitcher
,
Pediatrics
77
,
281
(
1986
).
5.
J.
Li
and
Y.
Lu
,
J. Am. Chem. Soc.
122
,
10466
(
2000
).
6.
D. A.
Skoog
,
Principles of Instrumental Analysis
(
Saunders
,
New York
,
1985
).
7.
S.
Curry
,
Vox Sang.
83
,
315
(
2002
).
8.
I.
Petitpas
,
A. A.
Bhattacharya
,
S.
Twine
,
M.
East
, and
S.
Curry
,
J. Biol. Chem.
276
,
22804
(
2001
).
9.
Y.
Yuan
,
Y.
Cai
,
Q.
Xie
, and
S.
Yao
,
Anal. Sci.
18
,
767
(
2002
).
10.
L.
Peng
,
X.
Hongyu
,
L.
Xi
,
R.
Lifang
, and
Z.
Chaocan
,
Biol. Trace Elem. Res.
118
,
97
(
2007
).
11.
A. F.
Zuluaga
,
U.
Utzinger
,
A.
Durkin
,
H.
Fuchs
,
A.
Gillenwater
,
R.
Jacob
,
B.
Kemp
,
J.
Fan
, and
R.
Richards-Kortum
,
Appl. Spectrosc.
53
,
302
(
1999
).
12.
M. L.
Nahorniak
and
K. S.
Booksh
,
Analyst (Cambridge, U.K.)
131
,
1308
(
2006
).
13.
J. R.
Lakowicz
and
I.
Gryczynski
,
Biophys. Chem.
45
,
1
(
1992
).
14.
F.
Fleury
,
A.
Ianoul
,
M.
Berjot
,
A.
Feofanov
,
A. J. P.
Alie
, and
I.
Nabiev
,
FEBS Lett.
411
,
215
(
1997
).
15.
K.
Oettl
and
R. E.
Stauber
,
Br. J. Pharmacol.
151
,
580
(
2007
).
16.
X. M.
He
and
D. C.
Carter
,
Nature (London)
358
,
209
(
1992
).
17.
R.
Glowacki
and
H.
Jakubowski
,
J. Biol. Chem.
279
,
10864
(
2004
).
18.
P. L.
Goering
,
Neurotoxicology
14
,
45
(
1993
).
19.
B. L.
Fletcher
and
A. L.
Tappel
,
Lipids
6
,
172
(
1971
).
20.
L.
Homchaudhuri
and
R.
Swaminathan
,
Chem. Lett.
30
,
844
(
2001
).
You do not currently have access to this content.