We report on sensitive and specific detection and quantification of a template in a molecularly imprinted polymer (MIP) using Raman microspectroscopy. The -blocking drug -propranolol and its enantiomer, -propranolol, were used as target molecules since the selectivity of this MIP is well established and serves as an appropriate validation standard. Specific peaks originating in the template were identified in the Raman spectrum, allowing quantification of bound target molecule. We demonstrate that label-free monitoring can be achieved from volumes as small as of MIP, based on a single identifying peak.
REFERENCES
1.
R.
Arshady
and K.
Mosbach
, Makromol. Chem.
182
, 687
(1981
).2.
3.
S. C.
Zimmerman
and N. G.
Lemcoff
, Chem. Commun.
2004
, 5
.4.
K.
Haupt
, Chem. Commun.
2003
, 171
.5.
V.
Espina
, A. I.
Mehta
, M. E.
Winters
, V.
Calvert
, J.
Wulfkuhle
, E. F.
Petricoin
, and L. A.
Liotta
, Proteomics
3
, 2091
(2003
).6.
A.
Lueking
, D. J.
Cahill
, and S.
Mullner
, Drug Discovery Today
10
, 789
(2005
).7.
M.
Lynch
, C.
Mosher
, J.
Huff
, S.
Nettikadan
, J.
Johnson
, and E.
Henderson
, Proteomics
4
, 1695
(2004
).8.
9.
A. -S.
Belmont
, M.
Sokuler
, K.
Haupt
, and L. A.
Gheber
, Appl. Phys. Lett.
90
, 193101
(2007
).10.
F.
Vandevelde
, T.
Leïchlé
, C.
Ayela
, C.
Bergaud
, L.
Nicu
, and K.
Haupt
, Langmuir
23
, 6490
(2007
).11.
M.
Jakusch
, M.
Janotta
, B.
Mizaikoff
, K.
Mosbach
, and K.
Haupt
, Anal. Chem.
71
, 4786
(1999
).12.
St.
Kostrewa
, M.
Emgenbroich
, D.
Klockow
, and G.
Wulff
, Macromol. Chem. Phys.
204
, 481
(2003
).13.
R. H.
Uibel
and J. M.
Harris
, Anal. Chem.
77
, 991
(2005
).14.
D.
McStay
, A. H.
Al-Obaidi
, R.
Hoskins
, and P. J.
Quinn
, J. Opt. A, Pure Appl. Opt.
7
, S340
(2005
).15.
L. I.
Andersson
, Anal. Chem.
68
, 111
(1996
).16.
R. H.
Schmidt
and K.
Haupt
, Chem. Mater.
17
, 1007
(2005
).© 2009 American Institute of Physics.
2009
American Institute of Physics
You do not currently have access to this content.