Ultrasonics signals at frequencies 5.7±0.1 and 6.8±0.1GHz are measured in two organelles of a single vegetal cell in vitro with a picosecond ultrasonic technique. Using standard values for cell optical index, ultrasound velocities of 1.6±0.1 and 2.0±0.1μmns are measured from several signals recorded in the vacuole and in the nucleus of a single Allium cepa cell, respectively. A 1μm lateral and 0.25μm depth resolution is attained.

1.
J.
Neto
,
U.
Agero
,
R.
Gazzinelli
, and
O.
Mesquita
,
Biophys. J.
91
,
1108
(
2006
).
2.
J.
Bereiter-Hahn
,
Mechanical Principles of Architecture of Eukaryotic Cell: In Cytomechanics
, edited by
J.
Bereiter-Hahn
,
R.
Anderson
, and
W.
Reif
(
Springer
,
Heidelberg, New York
,
1987
), p.
1
.
3.
A.
Simon
and
M. C.
Durrieu
,
Micron
37
,
1
(
2006
).
4.
A.
Pierres
,
A. M.
Benoliel
, and
P.
Bongrand
,
J. Biol. Chem.
270
,
26586
(
1995
).
5.
N.
Peterson
,
W.
McConnaughey
, and
E.
Elson
,
Proc. Natl. Acad. Sci. U.S.A.
79
,
5327
(
1982
).
6.
A.
Young
and
E.
Evans
,
Biophys. J.
56
,
139
(
1989
).
7.
A.
Zilker
,
H.
Engelhardt
, and
E.
Sackmann
,
J. Phys. (France)
48
,
2139
(
1987
).
8.
A.
Ashkin
and
J.
Dziedzic
,
Proc. Natl. Acad. Sci. U.S.A.
86
,
7914
(
1989
).
9.
P.
Valberg
and
H.
Feldman
,
Biophys. J.
52
,
551
(
1987
).
10.
M.
Radmacher
,
M.
Fritz
,
C.
Kacher
,
J.
Cleveland
, and
P.
Hansma
,
Biophys. J.
70
,
556
(
1994
).
11.
D. M.
Gryte
,
M. D.
Ward
, and
W. S.
Hu
,
Biotechnol. Prog.
9
,
105
(
1993
).
12.
M.
Horoyan
,
A. M.
Benoliel
,
C.
Capo
, and
P.
Bongrand
,
Cell Biophys.
17
,
243
(
1990
).
13.
J.
Hildebrand
,
D.
Rugar
,
R. N.
Johnston
, and
C. F.
Quate
,
Proc. Natl. Acad. Sci. U.S.A.
78
,
1656
(
1981
).
14.
B.
Hadimioglu
and
J. S.
Foster
,
J. Appl. Phys.
56
,
1976
(
1984
).
15.
A.
Korpel
,
L. W.
Kessler
, and
P. R.
Palermo
,
Nature (London)
232
,
110
(
1971
).
16.
T.
Kundu
,
J. P.
Lee
,
C.
Blase
, and
J.
Bereiter-Hahn
,
J. Acoust. Soc. Am.
120
,
1646
(
2006
).
17.
B. R.
Tittmann
,
C.
Miyasaka
,
A. M.
Mastro
, and
R. R.
Mercer
,
IEEE Trans. Ultrason. Ferroelectr. Freq. Control
54
,
1502
(
2007
).
18.
C.
Thomsen
,
H. T.
Grahn
,
H. J.
Maris
, and
J.
Tauc
,
Phys. Rev. B
34
,
4129
(
1986
).
19.
G.
Tas
and
H. J.
Maris
,
Phys. Rev. B
55
,
1852
(
1997
).
20.
L. J.
Shelton
,
F.
Yang
,
W. K.
Ford
, and
H. J.
Maris
,
Phys. Status Solidi B
242
,
1379
(
2005
).
21.
P. L.
Kuo
,
S. K.
Ghosh
,
W. J.
Liang
, and
Y. T.
Hsien
,
J. Polym. Sci. A
39
,
3018
(
2001
).
22.
M.
Long
and
H. J.
Rack
,
Biomaterials
19
,
1621
(
1998
).
23.
F.
Guillemot
,
F.
Prima
,
R.
Bareille
,
D.
Gordin
,
T.
Gloriant
,
M. C.
Porte-Durrieu
,
D.
Ansel
, and
Ch.
Baquey
,
Med. Biol. Eng. Comput.
42
,
137
(
2004
).
24.
V. A.
Grosso
and
C. W.
Mader
,
J. Acoust. Soc. Am.
52
,
1442
(
1972
).
25.
J.
Beuthan
,
O.
Minet
,
J.
Helfmann
,
M.
Herrig
, and
G.
Muller
,
Phys. Med. Biol.
41
,
369
(
1996
).
26.
R.
Cote
and
A.
Devos
,
Rev. Sci. Instrum.
76
,
1
(
2005
).
27.
C.
Rossignol
,
B.
Perrin
,
S.
Laborde
,
L.
Vandenbulcke
,
M. I.
De Barros
, and
P.
Djemia
,
J. Appl. Phys.
95
,
4157
(
2004
).
28.
A. G.
Every
and
A.
McCardy
,
Numerical Data and Fundamental Relationships in Science and Technology
,
Landolt-Bornstein, New Series
, Group III Vol.
29A
(
Springer
,
Berlin
,
1992
).
29.
The value of the optical index of the different organelles of a living cell is still an open question and it is out the objective of this letter. Several papers have shown that the optical index of the cytoplasm and the nucleus are very similar and both are close to 1.4. See for instance,
C. L.
Curl
,
C. J.
Bellair
,
T.
Harris
,
B. E.
Allman
,
P. J.
Harris
,
A. G.
Stewart
,
A.
Roberts
,
K. A.
Nugent
, and
L. M. D.
Delbridge
,
Cytometry, Part A
65A
,
88
(
2005
);
W. Z.
Song
,
X. M.
Zhang
,
A. Q.
Liu
,
C. S.
Lim
,
P. H.
Yap
, and
H. M. M.
Hosseini
,
Appl. Phys. Lett.
89
,
203901
(
2006
);
B.
Beauvoit
,
T.
Kitai
, and
B.
Chance
,
Biophys. J.
67
,
2501
(
1994
);
[PubMed]
R.
Drezek
,
A.
Dunn
, and
R.
Richards-Kortum
,
Opt. Express
6
,
147
(
2000
).
[PubMed]
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