An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.
REFERENCES
1.
2.
3.
J. H.
Li
, L.
Theofanous
, S.
Stickel
, H.
Bouton-Verville
, K. E.
Burgin
, S.
Jakubchak
, T. E.
Wagner
, and Y. Z.
Wei
, Int. J. Oncol.
31
, 193
(2007
).4.
Y. C.
Wei
, R. P.
Sticca
, J.
Li
, L. M.
Holmes
, K. E.
Burgin
, S.
Jakubchak
, H.
Bouton-Verville
, J.
Williamson
, K.
Meyer
, L.
Evans
, J.
Martin
, J. J.
Stephenson
, S.
Trocha
, S.
Smith
, and T. E.
Wagner
, Oncol. Rep.
18
, 665
(2007
).5.
Y. Z.
Wei
, R. P.
Sticca
, L. M.
Holmes
, K. E.
Burgin
, J. H.
Li
, J.
Williamson
, L.
Evans
, S. J.
Smith
, J. J.
Stephenson
, and T. E.
Wagner
, Int. J. Oncol.
28
, 585
(2006
).6.
T.
Kaneta
, Y.
Ishidzu
, N.
Mishima
, and T.
Imasaka
, Anal. Chem.
69
, 2701
(1997
).7.
M. P.
MacDonald
, G. C.
Spalding
, and K.
Dholakia
, Nature (London)
426
, 421
(2003
).8.
M. M.
Wang
, C. A.
Schnabel
, M.
Chachisvilis
, R.
Yang
, M. J.
Paliotti
, L. A.
Simons
, L.
McMullin
, N.
Hagen
, K.
Lykstad
, E.
Tu
, L. M.
Pestana
, S.
Sur
, H. C.
Zhang
, W. F.
Butler
, I.
Kariv
, and P. J.
Marchand
, Appl. Opt.
42
, 5765
(2003
).9.
J.
Guck
, S.
Schinkinger
, B.
Lincoln
, F.
Wottawah
, S.
Ebert
, M.
Romeyke
, D.
Lenz
, H. M.
Erickson
, R.
Ananthakrishnan
, D.
Mitchell
, J.
Kas
, S.
Ulvick
, and C.
Bilby
, Biophys. J.
88
, 3689
(2005
).10.
Y. Y.
Sun
, L. S.
Ong
, and X. C.
Yuan
, Appl. Phys. Lett.
89
, 141108
(2006
).11.
Y. Y.
Sun
, X. C.
Yuan
, L. S.
Ong
, J.
Bu
, S. W.
Zhu
, and R.
Liu
, Appl. Phys. Lett.
90
, 031107
(2007
).12.
13.
Y. K.
Nahmias
, B. Z.
Gao
, and D. J.
Odde
, Appl. Opt.
43
, 3999
(2004
).14.
15.
M.
Sridharan
, M.S. Thesis, Clemson University
, 2006
.16.
K.
Svoboda
and S. M.
Block
, Annu. Rev. Biophys. Biomol. Struct.
23
, 247
(1994
).17.
See EPAPS Document No. E-APPLAB-92-045822 for a movie file of laser guidance for speed comparison between a TC-1 regular cell (left column) and an L-10 modified cell (right column). For more information on EPAPS, see http://www.aip.org/pubservs/epaps.html.
© 2008 American Institute of Physics.
2008
American Institute of Physics
You do not currently have access to this content.
