The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam.

1.
A.
Vogel
,
J.
Noack
,
G.
Huttman
, and
G.
Paltauf
,
Appl. Phys. Lett.
81
,
1015
(
2005
).
2.
U. K.
Tirlapur
and
K.
Konig
,
Nature (London)
418
,
290
(
2002
).
3.
D.
Stevenson
,
B.
Agate
,
X.
Tsampoula
,
P.
Fischer
,
C. T. A.
Brown
,
W.
Sibbett
,
A.
Riches
,
F.
Gunn-Moore
, and
K.
Dholakia
,
Opt. Express
14
,
7125
(
2006
).
4.
D.
McGloin
and
K.
Dholakia
,
Contemp. Phys.
46
,
15
(
2005
).
5.
P.
Dufour
,
M.
Piche
,
Y.
De Koninck
, and
N.
McCarthy
,
Appl. Opt.
45
,
9246
(
2006
).
6.
J.
Durnin
,
J. Opt. Soc. Am. A
4
,
651
(
1987
).
7.
L.
Paterson
,
B.
Agate
,
M.
Comrie
,
R.
Ferguson
,
T. K.
Lake
,
J. E.
Morris
,
A. E.
Carruthers
,
C. T. A.
Brown
,
W.
Sibbett
,
P. E.
Bryant
,
F.
Gunn-Moore
,
A. C.
Riches
, and
K.
Dholakia
,
Opt. Express
13
,
595
(
2005
).
8.
V.
Garcés-Chávez
,
D.
McGloin
,
H.
Melville
,
W.
Sibbett
, and
K.
Dholakia
,
Nature (London)
419
,
145
(
2002
).
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