This work presents a simple process of reflow of melted glass by laser to form an hourglass-shaped aperture for cellular electrophysiological study. The fabricated aperture proves to be smooth, circular, debris-free, and freshly activated. Two-phase flow simulation illuminates details of the reflow process. The resulting seal resistance quality is well suited to measure ion-channel activities; a seal resistance of for PC-12 cell and a typical resistance of for Chinese hamster ovary cells were achieved. This approach can be integrated with microfluidics in a single cell patch or array configuration.
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