The authors report a technique which produces cell-to-cell electrofusion using a common direct current power supply on a microfluidic platform. In the authors’ method, the cells were first conjugated based on biotin-streptavidin interaction. The electrofusion was then conducted by flowing the linked cells through a simple microfluidic channel with geometric variation under continuous direct current voltage. This microfluidics-based technique offers processing at the level of single cell pairs with efficiency comparable to that of conventional electrofusion technique based on electrical pulses.
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See EPAPS Document No.E-APPLAB-89-205649 for the simulation and experimental protocols involved in this study. The document can be reached via a direct link in the online article’s HTML reference section or via the EPAPS homepage (http://www.aip.org/pubservs/epaps.html/.
© 2006 American Institute of Physics.
2006
American Institute of Physics
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