We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.
REFERENCES
1.
S.
Miltenyi
, W.
Müller
, W.
Weichel
, and A.
Radbruch
, Cytometry
11
, 231
(1990
).2.
A. B.
Kantor
, I.
Gibbons
, S.
Miltenyi
, and J.
Schmitz
, Magnetic Cell Sorting with Colloidal Superparamagnetic Particles in Cell Separation Methods and Applications
, edited by D.
Recktenwald
and A.
Radbruch
(Marcel Dekker
, New York
, 1998
), p. 153
.3.
J. J.
Chalmers
, M.
Zborowski
, L.
Sun
, and L.
Moore
, Biotechnol. Prog.
14
, 141
(1998
).4.
C. H.
Ahn
, M. G.
Allen
, W.
Trimmer
, Y.
Jun
, and S.
Erramilli
, J. Microelectromech. Syst.
5
, 1057
(1996
).5.
J.
Choi
, C. H.
Ahn
, S.
Bhansali
, and H. T.
Henderson
, Sens. Actuators B
68
, 34
(2000
).6.
N.
Darnton
, O.
Bakajin
, R.
Huang
, B.
North
, J. O.
Tegenfeldt
, E. C.
Cox
, J.
Sturm
, and R. H.
Austin
, J. Phys.: Condens. Matter
13
, 4891
(2001
).7.
A.
Bikoue
, G.
Jonossy
, and D.
Barnett
, J. Immunol. Methods
266
, 219
(2002
).8.
K. E.
McCloskey
, J. H.
Chalmers
, and M.
Zborowski
, Anal. Chem.
75
, 6868
(2003
).9.
T.
McPherson
, A.
Kidane
, I.
Szleifer
, and K.
Park
, Langmuir
14
, 176
(1998
).© 2004 American Institute of Physics.
2004
American Institute of Physics
You do not currently have access to this content.
