Electroacoustic imaging is an imaging modality used to detect electric field energy distribution during electroporation, offering valuable guidance for clinical procedures, particularly in deep tissues. Traditionally, single-element piezoelectric transducers or arrays have been employed for this purpose. However, these piezoelectric sensors are sensitive to electromagnetic interference and require physical contact with the sample through a coupling medium, raising concerns for both clinical and preclinical applications. To overcome these limitations, a multi-channel random quadrature ultrasonics system has been developed, enabling non-contact detection of electroacoustic signals. In this study, we demonstrated that this non-contact technique effectively detects electroacoustic signals, identifies electroporation regions, and reconstructs electric energy distribution, offering a promising approach for monitoring electroporation therapy.

1.
H. A.
Whitaker
,
C. U. M.
Smith
, and
S.
Finger
,
Brain, Mind, and Medicine: Essays in Eighteenth-Century Neuroscience
(
Springer
,
New York, NY
,
2007
).
2.
F. X.
Hart
and
J. R.
Palisano
,
The Application of Electric Fields in Biology and Medicine
(
InTech
,
2018
).
3.
D.
Fabian
,
M. D. P.
Guillermo Prieto Eibl
,
I.
Alnahhas
,
N.
Sebastian
,
P.
Giglio
,
V.
Puduvalli
,
J.
Gonzalez
, and
J. D.
Palmer
, “
Treatment of glioblastoma (GBM) with the addition of tumor-treating fields (TTF): A review
,”
Cancers
11
,
174
(
2019
).
4.
C. N. M.
Ryan
,
M. N.
Doulgkeroglou
,
D. I.
Zeugolis
,
C. N. M.
Ryan
,
M. N.
Doulgkeroglou
, and
D. I.
Zeugolis
, “
Electric field stimulation for tissue engineering applications
,”
BMC Biomed. Eng.
3
,
1
9
(
2021
).
5.
M. A.
Messerli
and
D. M.
Graham
, “
Extracellular electrical fields direct wound healing and regeneration
,”
Biol. Bull.
221
,
79
(
2011
).
6.
H. J.
Scheffer
,
K.
Nielsen
,
M.
Jong
,
A.
Tilborg
,
J. M.
Vieveen
,
A. R. A.
Bouwman
,
S.
Meijer
,
C.
Kuijk
,
P.
Tol
, and
M. R.
Meijerink
, “
Irreversible electroporation for nonthermal tumor ablation in the clinical setting: A systematic review of safety and efficacy
,”
J. Vasc. Interventional Radiol.
25
,
997
(
2014
).
7.
N.
Bhutiani
,
S.
Agle
,
Y.
Li
,
S.
Li
, and
R. C. G.
Martin
, “
Irreversible electroporation enhances delivery of gemcitabine to pancreatic adenocarcinoma
,”
J. Surg. Oncol.
114
,
181
(
2016
).
8.
A.
Taketo
, “
DNA transfection of Escherichia coli by electroporation
,”
Biochim. Biophys. Acta (BBA) - Gene Struct. Expression
949
,
318
(
1988
).
9.
J. C.
Weaver
, “
Electroporation theory. Concepts and mechanisms
,”
Methods Mol. Biol.
55
,
3
28
(
1995
).
10.
A. R.
Ruiz-Fernández
,
L.
Campos
,
S. E.
Gutierrez-Maldonado
,
G.
Núñez
,
F.
Villanelo
,
T.
Perez-Acle
,
A. R.
Ruiz-Fernández
,
L.
Campos
,
S. E.
Gutierrez-Maldonado
,
G.
Núñez
,
F.
Villanelo
, and
T.
Perez-Acle
, “
Nanosecond pulsed electric field (nsPEF): Opening the biotechnological Pandora's box
,”
Int. J. Mol. Sci.
23
,
6158
(
2022
).
11.
E.
Gudvangen
,
V.
Kim
,
V.
Novickij
,
F.
Battista
,
A. G.
Pakhomov
,
E.
Gudvangen
,
V.
Kim
,
V.
Novickij
,
F.
Battista
, and
A. G.
Pakhomov
, “
Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation
,”
Sci. Rep.
12
,
1763
(
2022
).
12.
C.
Valdez
,
M. B.
Jirjis
,
C. C.
Roth
,
R. A.
Barnes
,
B. L.
Ibey
,
C.
Valdez
,
M. B.
Jirjis
,
C. C.
Roth
,
R. A.
Barnes
, and
B. L.
Ibey
, “
Nanosecond electric pulses modulate skeletal muscle calcium dynamics and contraction
,”
Proc. SPIE
10066
,
100660X
(
2017
).
13.
T. F.
Justesen
,
A.
Orhan
,
H.
Raskov
,
C.
Nolsoe
, and
I.
Gögenur
, “
Electroporation and immunotherapy—Unleashing the abscopal effect
,”
Cancers
14
,
2876
(
2022
).
14.
A.
Vižintin
,
S.
Marković
,
J.
Ščančar
, and
D.
Miklavčič
, “
Electroporation with nanosecond pulses and bleomycin or cisplatin results in efficient cell kill and low metal release from electrodes
,”
Bioelectrochemistry
140
,
107798
(
2021
).
15.
M.
Xu
,
D.
Xu
,
G.
Dong
,
Z.
Ren
,
W.
Zhang
,
T.
Aji
,
Q.
Zhao
,
X.
Chen
, and
T.
Jiang
, “
The safety and efficacy of nanosecond pulsed electric field in patients with hepatocellular carcinoma: A prospective phase 1 clinical study protocol
,”
Front. Oncol.
12
,
869316
(
2022
).
16.
M.
Silk
,
D.
Tahour
,
G.
Srimathveeravalli
,
S. B.
Solomon
, and
R. H.
Thornton
, “
The state of irreversible electroporation in interventional oncology
,”
Semin. Interventional Radiol.
31
,
111
(
2014
).
17.
Y.
Xu
,
L.
Sun
,
S.
Wang
,
Y.
Yan
,
P.
Pandey
,
V.
Novickij
,
L.
Xiang
,
Y.
Xu
,
L.
Sun
,
S.
Wang
,
Y.
Yan
,
P.
Pandey
,
V.
Novickij
, and
L.
Xiang
, “
Electroacoustic tomography for real-time visualization of electrical field dynamics in deep tissue during electroporation
,”
Commun. Eng.
2
(
1
),
75
(
2023
).
18.
Z.
Hosseinaee
,
M.
Le
,
K.
Bell
, and
P. H.
Reza
, “
Towards non-contact photoacoustic imaging [review]
,”
Photoacoustics
20
,
100207
(
2020
).
19.
J.
Sirohi
and
I.
Chopra
, “
Fundamental understanding of piezoelectric strain sensors
,”
J. Intell. Mater. Syst. Struct.
11
,
246
257
(
2000
).
20.
J. W.
Wagner
, “
Optical detection of ultrasound
,”
Phys. Acoust.
19
,
201
266
(
1990
).
21.
G.
Wissmeyer
,
M. A.
Pleitez
,
A.
Rosenthal
, and
V.
Ntziachristos
, “
Looking at sound: Optoacoustics with all-optical ultrasound detection
,”
Light-Sci. Appl.
7
,
53
(
2018
).
22.
R. A.
Barnes
,
C. C.
Roth
,
H. T.
Beier
,
G.
Noojin
,
C.
Valdez
,
J.
Bixler
,
E.
Moen
,
M.
Shadaram
, and
B. L.
Ibey
, “
Probe beam deflection optical imaging of thermal and mechanical phenomena resulting from nanosecond electric pulse (nsEP) exposure
,”
Opt. Express
25
,
6621
6643
(
2017
).
23.
C.
Merla
,
M.
Liberti
,
P.
Marracino
,
A.
Muscat
,
A.
Azan
,
F.
Apollonio
,
L. M.
Mir
,
C.
Merla
,
M.
Liberti
,
P.
Marracino
,
A.
Muscat
,
A.
Azan
,
F.
Apollonio
, and
L. M.
Mir
, “
A wide-band bio-chip for real-time optical detection of bioelectromagnetic interactions with cells
,”
Sci. Rep.
8
,
5044
(
2018
).
24.
C.
Merla
,
M.
Nardoni
,
M.
Scherman
,
S.
Petralito
,
L.
Caramazza
,
F.
Apollonio
,
M.
Liberti
,
P.
Paolicelli
,
B.
Attal-Tretout
, and
L. M.
Mir
, “
Changes in hydration of liposome membranes exposed to nanosecond electric pulses detected by wide-field coherent anti-stokes Raman microspectroscopy
,”
Bioelectrochemistry
147
,
108218
(
2022
).
25.
B.
Pouet
,
S.
Breugnot
, and
P.
Clémenceau
, “
Robust laser‐ultrasonic interferometer based on random quadrature demodulation
,”
AIP Conf. Proc.
820
,
233
239
(
2006
).
26.
Z.
Jiang
,
Y.
Xu
,
L.
Sun
,
S.
Srinivasan
,
Q. J.
Wu
,
L.
Xiang
, and
L.
Ren
, “
Enhanced electroacoustic tomography with supervised learning for real‐time electroporation monitoring
,”
Precis. Radiat. Oncol.
8
,
110
(
2024
).
27.
B.
Pouet
,
A.
Wartelle
, and
S.
Breugnot
, “
Multi-channel random-quadrature receiver for industrial laser-ultrasonics
,” in
2011 IEEE International Ultrasonics Symposium
(IEEE,
2011
).
28.
Y.
Zhao
,
H.
Liu
,
S. P.
Bhonsle
,
Y.
Wang
,
R. V.
Davalos
,
C.
Yao
,
Y.
Zhao
,
H.
Liu
,
S. P.
Bhonsle
,
Y.
Wang
,
R. V.
Davalos
, and
C.
Yao
, “
Ablation outcome of irreversible electroporation on potato monitored by impedance spectrum under multi-electrode system
,”
Biomed. Eng. Online
17
,
1
13
(
2018
).
29.
S.
Jeong
,
H.
Kim
,
J.
Park
,
K. W.
Kim
,
S. B.
Sim
,
J. H.
Chung
,
S.
Jeong
,
H.
Kim
,
J.
Park
,
K. W.
Kim
,
S. B.
Sim
, and
J. H.
Chung
, “
Evaluation of electroporated area using 2,3,5-triphenyltetrazolium chloride in a potato model
,”
Sci. Rep.
11
,
20431
(
2021
).
30.
M.
Xu
and
L. V.
Wang
, “
Universal back-projection algorithm for photoacoustic computed tomography
,”
Phys. Rev. E
71
,
016706
(
2005
).
31.
M.
Wang
,
A.
Zarafshani
,
P.
Samant
,
J.
Merrill
,
D.
Li
, and
L.
Xiang
, “
Feasibility of electroacoustic tomography: A simulation study
,”
IEEE Trans. Ultrason. Ferroelectr. Freq. Control
67
,
889
(
2020
).
32.
X.
Zhang
,
J. R.
Fincke
,
C. M.
Wynn
,
M. R.
Johnson
,
R. W.
Haupt
, and
B. W.
Anthony
, “
Full noncontact laser ultrasound: First human data
,”
Light. Sci. Appl.
8
,
119
(
2019
).
33.
F.
Guo
,
X. H.
Gou
,
J. G.
Sun
,
J.
Hong
, and
Y. P.
Zhang
, “
Modeling methods in overlapping electroporation treatments: Pulse number effects on tissue conductivity and ablation area
,”
Electrochim. Acta
503
,
144883
(
2024
).
34.
H.
Li
,
B.
Dong
,
Z.
Zhang
,
H. F.
Zhang
, and
C.
Sun
, “
A transparent broadband ultrasonic detector based on an optical micro-ring resonator for photoacoustic microscopy
,”
Sci. Rep.
4
,
4496
(
2014
).
35.
A.
Rosenthal
,
D.
Razansky
, and
V.
Ntziachristos
, “
High-sensitivity compact ultrasonic detector based on a pi-phase-shifted fiber Bragg grating
,”
Opt. Lett.
36
,
1833
1835
(
2011
).
36.
E.
Zhang
,
J.
Laufer
, and
P.
Beard
, “
Backward-mode multiwavelength photoacoustic scanner using a planar Fabry-Perot polymer film ultrasound sensor for high-resolution three-dimensional imaging of biological tissues
,”
Appl. Opt.
47
,
561
577
(
2008
).
37.
R.
van Bergen
,
L.
Sun
,
P. K.
Pandey
,
S.
Wang
,
K.
Bjegovic
,
G.
Gonzalez
,
Y.
Chen
,
R.
Lopata
, and
L.
Xiang
, “
Discrete wavelet transformation for the sensitive detection of ultrashort radiation pulse with radiation-induced acoustics
,”
IEEE Trans. Radiat. Plasma Med. Sci.
8
,
76
87
(
2024
).
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