Nanothermometry is crucial for understanding physical, chemical, and biological systems, which require precise temperature measurement. Fluorescent nanodiamonds containing nitrogen-vacancy (NV) color centers offer an approach to temperature sensing. In this study, we present the spectrofluorometric characteristics of the NV zero-phonon lines (575 and 637 nm), in 100 nm nanodiamonds in aqueous volume ensembles at a concentration of 0.5 mg/ml, across the temperature range of 30–45 °C. The NV0 and NV− fluorescence intensities achieved high linear correlation values of 0.99 (INV0) and 0.94 (INV−), respectively, demonstrating their efficiency in high precision temperature assessment. Additionally, we explore NV0 width as temperature increases, NV populations intensity ratios, and INV0/INV− ratios to gain insights into thermal quenching phenomena in fluorescent nanodiamonds, where upon heating, an increasing trend for INV−/(INV0+INV−) is observed, while an antisymmetric effect takes place for INV0/(INV0+INV−). These findings indicate the potential of commercial nanodiamonds for precise all-optical fluorescence-based temperature sensing.
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