Quantum diamond microscopy is an emerging versatile technique for studying the magnetic properties of materials. It has been applied extensively in condensed matter physics and materials science and has blossomed into a unique platform for the magnetic study of biological systems. To date, biological demonstrations of quantum diamond microscopy have been performed under ambient conditions. Here, we extend this magnetic microscopy platform to cryogenic temperatures to study magnetic anisotropy and the blocking temperature from an individual iron organelle found within the inner ear of pigeons. Our work confirms that the interface between thin histological tissue sections and diamond can be maintained under cryogenic temperatures. Our magnetic images provide evidence of magnetic anisotropy from a single iron organelle with sub-cellular resolution using this correlative optical imaging method. This approach may be extended to a broad range of systems where magnetic materials play structural and functional roles in biological systems.
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16 January 2023
Research Article|
January 18 2023
Temperature and angle dependent magnetic imaging of biological iron nanoparticles using quantum diamond microscopy
R. W. de Gille
;
R. W. de Gille
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Writing – original draft, Writing – review & editing)
1
Department of Physics, University of Melbourne
, Parkville, Victoria 3010, Australia
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A. J. Healey
;
A. J. Healey
(Data curation, Investigation, Methodology, Writing – review & editing)
1
Department of Physics, University of Melbourne
, Parkville, Victoria 3010, Australia
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I. O. Robertson
;
I. O. Robertson
(Data curation, Investigation, Methodology, Writing – review & editing)
2
School of Science, RMIT University
, Melbourne, Victoria 3001, Australia
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L. T. Hall
;
L. T. Hall
(Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – review & editing)
3
Department of Chemistry, University of Melbourne
, Parkville, Victoria 3010, Australia
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J.-P. Tetienne
;
J.-P. Tetienne
(Data curation, Funding acquisition, Investigation, Methodology, Resources, Writing – review & editing)
2
School of Science, RMIT University
, Melbourne, Victoria 3001, Australia
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E. P. Malkemper
;
E. P. Malkemper
(Conceptualization, Data curation, Investigation, Methodology, Resources, Writing – review & editing)
4
Max Planck Research Group Neurobiology of Magnetoreception, Max Planck Institute for Neurobiology of Behavior–caesar
, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany
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D. A. Keays
;
D. A. Keays
(Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Resources, Writing – review & editing)
5
Institute for Molecular Pathology
, Campus Vienna Biocenter 1, Vienna 1030, Austria
6
Department of Anatomy and Neuroscience, University of Melbourne
, Parkville, Victoria 3010, Australia
7
Division of Neurobiology, Department Biology II, Ludwig-Maximilians-University Munich
, Planegg-Martinsried, Munich 82152, Germany
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L. C. L. Hollenberg
;
L. C. L. Hollenberg
(Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing)
1
Department of Physics, University of Melbourne
, Parkville, Victoria 3010, Australia
8
Centre for Quantum Computation and Communication Technology, University of Melbourne
, Parkville, Victoria 3010, Australia
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D. A. Simpson
D. A. Simpson
a)
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing)
1
Department of Physics, University of Melbourne
, Parkville, Victoria 3010, Australia
a)Author to whom correspondence should be addressed: simd@unimelb.edu.au
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a)Author to whom correspondence should be addressed: simd@unimelb.edu.au
Appl. Phys. Lett. 122, 032404 (2023)
Article history
Received:
July 26 2022
Accepted:
December 29 2022
Citation
R. W. de Gille, A. J. Healey, I. O. Robertson, L. T. Hall, J.-P. Tetienne, E. P. Malkemper, D. A. Keays, L. C. L. Hollenberg, D. A. Simpson; Temperature and angle dependent magnetic imaging of biological iron nanoparticles using quantum diamond microscopy. Appl. Phys. Lett. 16 January 2023; 122 (3): 032404. https://doi.org/10.1063/5.0114998
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