Three-dimensional (3D) fluorescence imaging is an essential technique in the biomedical field. In particular, 3D fluorescence imaging through dynamic scattering media is a crucial task for the minimally invasive observation of labeled cells. In this study, this task was accomplished via motionless optical scanning holography, proposed as a single-pixel 3D imaging technique. The proposed method does not require additional computational processing or optical components when the detected intensities do not considerably fluctuate irrespective of the presence of dynamic scattering media. The results of a proof-of-principle experiment indicated that the proposed method can help in computationally refocusing fluorescent objects that are placed at different positions behind dynamic scattering media.
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18 October 2021
Research Article|
October 18 2021
Three-dimensional fluorescence imaging through dynamic scattering media by motionless optical scanning holography
Naru Yoneda
;
Naru Yoneda
a)
1
Graduate School of Systems Engineering, Wakayama University
, 930 Sakaedani, Wakayama 640-8510, Japan
2
Japan Society for the Promotion of Science
, 5-3-1 Kojimachi, Chiyoda, Tokyo 102-0083, Japan
a)Author to whom correspondence should be addressed: s182063@wakayama-u.ac.jp
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Yusuke Saita
;
Yusuke Saita
3
Faculty of Systems Engineering, Wakayama University
, 930 Sakaedani, Wakayama 640-8510, Japan
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Takanori Nomura
Takanori Nomura
3
Faculty of Systems Engineering, Wakayama University
, 930 Sakaedani, Wakayama 640-8510, Japan
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a)Author to whom correspondence should be addressed: s182063@wakayama-u.ac.jp
Appl. Phys. Lett. 119, 161101 (2021)
Article history
Received:
August 10 2021
Accepted:
October 02 2021
Citation
Naru Yoneda, Yusuke Saita, Takanori Nomura; Three-dimensional fluorescence imaging through dynamic scattering media by motionless optical scanning holography. Appl. Phys. Lett. 18 October 2021; 119 (16): 161101. https://doi.org/10.1063/5.0066358
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