Structured illumination microscopy (SIM) achieves doubled spatial resolution through exciting the specimen with high-contrast, high-frequency sinusoidal patterns. Such an illumination pattern can be generated by laser interference or incoherent structured patterns. Opto-electronic devices, such as a Spatial Light Modulator (SLM) or a Digital Micro-mirror Device (DMD), can provide rapid switch of illumination patterns for SIM. Although the DMD is much more cost-effective than the SLM, it was previously restricted in association with incoherent light sources, as its diffractive orders are related to the incident angle and the wavelength of coherent incidence. To extend its application with coherent illumination, here, we model the DMD as a blazed grating and simulate the effect with DMD pattern changes in the SIM. With careful analysis of the illumination contrast along different angles and phases, we report a fast, high-resolution, and cost-efficient SIM with DMD modulation. Our home-built laser interference-based DMD-SIM (LiDMD-SIM) reveals the nuclear pore complex and microtubule in mammalian cells with doubled spatial resolution. We further proposed the multi-color LiDMD-SIM concept by jointly employing the DMD ON/OFF states with different incident angles for different wavelengths, with high contrast and maximum resolution enhancement.
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8 June 2020
Research Article|
June 09 2020
Structured illumination microscopy using digital micro-mirror device and coherent light source
Meiqi Li
;
Meiqi Li
a)
1
Department of Biomedical Engineering, College of Engineering, Peking University
, Beijing 100871, China
a)Authors to whom correspondence should be addressed: karl.hao.zhang@gmail.com and xipeng@pku.edu.cn
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Yaning Li
;
Yaning Li
1
Department of Biomedical Engineering, College of Engineering, Peking University
, Beijing 100871, China
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Wenhui Liu
;
Wenhui Liu
2
Department of Automation, Tsinghua University
, Beijing 100084, China
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Amit Lal
;
Amit Lal
3
Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
, Shenzhen 518055, Guangdong, China
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Shan Jiang
;
Shan Jiang
1
Department of Biomedical Engineering, College of Engineering, Peking University
, Beijing 100871, China
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Dayong Jin
;
Dayong Jin
3
Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
, Shenzhen 518055, Guangdong, China
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Houpu Yang
;
Houpu Yang
4
Breast Center, Peking University People's Hospital
, Beijing 100044, China
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Shu Wang
;
Shu Wang
4
Breast Center, Peking University People's Hospital
, Beijing 100044, China
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Karl Zhanghao
;
Karl Zhanghao
a)
1
Department of Biomedical Engineering, College of Engineering, Peking University
, Beijing 100871, China
3
Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
, Shenzhen 518055, Guangdong, China
a)Authors to whom correspondence should be addressed: karl.hao.zhang@gmail.com and xipeng@pku.edu.cn
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Peng Xi
Peng Xi
a)
1
Department of Biomedical Engineering, College of Engineering, Peking University
, Beijing 100871, China
3
Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology
, Shenzhen 518055, Guangdong, China
a)Authors to whom correspondence should be addressed: karl.hao.zhang@gmail.com and xipeng@pku.edu.cn
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a)Authors to whom correspondence should be addressed: karl.hao.zhang@gmail.com and xipeng@pku.edu.cn
Appl. Phys. Lett. 116, 233702 (2020)
Article history
Received:
March 21 2020
Accepted:
May 15 2020
Connected Content
A companion article has been published:
Digital mirror device combined with microscopy technique illuminates biological mechanisms
Citation
Meiqi Li, Yaning Li, Wenhui Liu, Amit Lal, Shan Jiang, Dayong Jin, Houpu Yang, Shu Wang, Karl Zhanghao, Peng Xi; Structured illumination microscopy using digital micro-mirror device and coherent light source. Appl. Phys. Lett. 8 June 2020; 116 (23): 233702. https://doi.org/10.1063/5.0008264
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