We developed a cryogenic objective mirror [Toratani-Fujiwara (TORA-FUJI) mirror] with a 36-μm field of view (FOV) radius and a 0.93 numerical aperture. The latest reported cryogenic objective mirror (INAGAWA mirror) under a superfluid-helium immersion condition had a nearly maximum numerical aperture (0.99) and was perfectly achromatic. However, its FOV radius was restricted to 1.5 μm, mainly due to coma aberration. In the TORA-FUJI mirror, correcting coma aberration realized the 36-μm FOV radius. In addition, the remaining four Seidel aberrations and the chromatic aberrations were sufficiently corrected. To evaluate the optical performance, the cryogenic fluorescence imaging of individual dyes was performed with the TORA-FUJI mirror at a 685-nm excitation wavelength. This result shows that the TORA-FUJI mirror in superfluid helium at 1.8 K exhibits nearly diffraction-limited performance in the FOV region.

Topics
Diffraction optics, Geometrical optics, Optical aberrations, Laser scanning, Dye, Fluorescence microscopy, Microscopy, Single molecule spectroscopy, Single molecule techniques, Laser microscopy
1.
G. W.
 
Li
 and
X. S.
 
Xie
,
Nature
 
475
(
7356
),
308
(
2011
).
https://doi.org/10.1038/nature10315
Google Scholar
Crossref
Search ADS
PubMed
 
2.
F.
 
Huang
,
G.
 
Sirinakis
,
E. S.
 
Allgeyer
,
L. K.
 
Schroeder
,
W. C.
 
Duim
,
E. B.
 
Kromann
,
T.
 
Phan
,
F. E.
 
Rivera-Molina
,
J. R.
 
Myers
,
I.
 
Irnov
,
M.
 
Lessard
,
Y. D.
 
Zhang
,
M. A.
 
Handel
,
C.
 
Jacobs-Wagner
,
C. P.
 
Lusk
,
J. E.
 
Rothman
,
D.
 
Toomre
,
M. J.
 
Booth
, and
J.
 
Bewersdorf
,
Cell
 
166
(
4
),
1028
(
2016
).
https://doi.org/10.1016/j.cell.2016.06.016
Google Scholar
Crossref
Search ADS
PubMed
 
3.
Y. W.
 
Chang
,
S. Y.
 
Chen
,
E. I.
 
Tocheva
,
A.
 
Treuner-Lange
,
S.
 
Loebach
,
L.
 
Sogaard-Andersen
, and
G. J.
 
Jensen
,
Nat. Methods
 
11
(
7
),
737
(
2014
).
https://doi.org/10.1038/nmeth.2961
Google Scholar
Crossref
Search ADS
PubMed
 
4.
R.
 
Kaufmann
,
P.
 
Schellenberger
,
E.
 
Seiradake
,
I. M.
 
Dobbie
,
E. Y.
 
Jones
,
I.
 
Davis
,
C.
 
Hagen
, and
K.
 
Grunewald
,
Nano Lett.
 
14
(
7
),
4171
(
2014
).
https://doi.org/10.1021/nl501870p
Google Scholar
Crossref
Search ADS
PubMed
 
5.
B.
 
Liu
,
Y. H.
 
Xue
,
W.
 
Zhao
,
Y.
 
Chen
,
C. Y.
 
Fan
,
L. S.
 
Gu
,
Y. D.
 
Zhang
,
X.
 
Zhang
,
L.
 
Sun
,
X. J.
 
Huang
,
W.
 
Ding
,
F.
 
Sun
,
W.
 
Ji
, and
T.
 
Xu
,
Sci. Rep.
 
5
,
13017
(
2015
).
https://doi.org/10.1038/srep13017
Google Scholar
Crossref
Search ADS
PubMed
 
6.
L.
 
Wang
,
B.
 
Bateman
,
L. C.
 
Zanetti-Domingues
,
A. N.
 
Moores
,
S.
 
Astbury
,
C.
 
Spindloe
,
M. C.
 
Darrow
,
M.
 
Romano
,
S. R.
 
Needham
,
K.
 
Beis
,
D. J.
 
Rolfe
,
D. T.
 
Clarke
, and
M. L.
 
Martin-Fernandez
,
Commun. Biol.
 
2
,
74
(
2019
).
https://doi.org/10.1038/s42003-019-0317-6
Google Scholar
Crossref
Search ADS
PubMed
 
7.
H.
 
Inagawa
,
Y.
 
Toratani
,
K.
 
Motohashi
,
I.
 
Nakamura
,
M.
 
Matsushita
, and
S.
 
Fujiyoshi
,
Sci. Rep.
 
5
,
12833
(
2015
).
https://doi.org/10.1038/srep12833
Google Scholar
Crossref
Search ADS
PubMed
 
8.
T.
 
Furubayashi
,
K.
 
Motohashi
,
K.
 
Wakao
,
T.
 
Matsuda
,
I.
 
Kii
,
T.
 
Hosoya
,
N.
 
Hayashi
,
M.
 
Sadaie
,
F.
 
Ishikawa
,
M.
 
Matsushita
, and
S.
 
Fujiyoshi
,
J. Am. Chem. Soc.
 
139
(
26
),
8990
(
2017
).
https://doi.org/10.1021/jacs.7b03899
Google Scholar
Crossref
Search ADS
PubMed
 
9.
S.
 
Fujiyoshi
,
M.
 
Fujiwara
,
C.
 
Kim
,
M.
 
Matsushita
,
A. M.
 
van Oijen
, and
J.
 
Schmidt
,
Appl. Phys. Lett.
 
91
(
5
),
3
(
2007
).
https://doi.org/10.1063/1.2767778
Google Scholar
Crossref
Search ADS
 
10.
S.
 
Fujiyoshi
,
M.
 
Fujiwara
, and
M.
 
Matsushita
,
Phys. Rev. Lett.
 
100
(
16
),
168101
(
2008
).
https://doi.org/10.1103/PhysRevLett.100.168101
Google Scholar
Crossref
Search ADS
PubMed
 
11.
S.
 
Fujiyoshi
,
M.
 
Hirano
,
M.
 
Matsushita
,
M.
 
Iseki
, and
M.
 
Watanabe
,
Phys. Rev. Lett.
 
106
(
7
),
078101
(
2011
).
https://doi.org/10.1103/PhysRevLett.106.078101
Google Scholar
Crossref
Search ADS
PubMed
 
12.
W. E.
 
Moerner
 and
D. P.
 
Fromm
,
Rev. Sci. Instrum.
 
74
(
8
),
3597
(
2003
).
https://doi.org/10.1063/1.1589587
Google Scholar
Crossref
Search ADS
 
13.
S.
 
Bolte
 and
F. P.
 
Cordelieres
,
J. Microsc.
 
224
,
213
(
2006
).
https://doi.org/10.1111/j.1365-2818.2006.01706.x
Google Scholar
Crossref
Search ADS
PubMed
 
14.
T.
 
Tanaami
,
S.
 
Otsuki
,
N.
 
Tomosada
,
Y.
 
Kosugi
,
M.
 
Shimizu
, and
H.
 
Ishida
,
Appl. Opt.
 
41
(
22
),
4704
(
2002
).
https://doi.org/10.1364/AO.41.004704
Google Scholar
Crossref
Search ADS
PubMed
 
15.
A.
 
Nakano
,
Cell Struct. Funct.
 
27
(
5
),
349
(
2002
).
https://doi.org/10.1247/csf.27.349
Google Scholar
Crossref
Search ADS
PubMed
 
16.
S.
 
Hayashi
 and
Y.
 
Okada
,
Mol. Biol. Cell
 
26
(
9
),
1743
(
2015
).
https://doi.org/10.1091/mbc.E14-08-1287
Google Scholar
Crossref
Search ADS
PubMed
 
17.
E. F.
 
Burton
,
Nature
 
140
,
1015
(
1937
).
https://doi.org/10.1038/1401015a0
Google Scholar
Crossref
Search ADS
 
18.
R. E.
 
Thompson
,
D. R.
 
Larson
, and
W. W.
 
Webb
,
Biophys. J.
 
82
(
5
),
2775
(
2002
).
https://doi.org/10.1016/S0006-3495(02)75618-X
Google Scholar
Crossref
Search ADS
PubMed
 
19.
H.
 
Tabe
,
K.
 
Sukenobe
,
T.
 
Kondo
,
A.
 
Sakurai
,
M.
 
Maruo
,
A.
 
Shimauchi
,
M.
 
Hirano
,
S.
 
Uno
,
M.
 
Kamiya
,
Y.
 
Urano
,
M.
 
Matsushita
, and
S.
 
Fujiyoshi
,
J. Phys. Chem. B
 
122
(
27
),
6906
(
2018
).
https://doi.org/10.1021/acs.jpcb.8b03977
Google Scholar
Crossref
Search ADS
PubMed
 
© 2019 Author(s).
2019
Author(s)

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