We propose and demonstrate a light-sheet-based 3D interrogation system on a microfluidic platform for screening biological specimens during flow. To achieve this, a diffraction-limited light-sheet (with a large field-of-view) is employed to optically section the specimens flowing through the microfluidic channel. This necessitates optimization of the parameters for the illumination sub-system (illumination intensity, light-sheet width, and thickness), microfluidic specimen platform (channel-width and flow-rate), and detection sub-system (camera exposure time and frame rate). Once optimized, these parameters facilitate cross-sectional imaging and 3D reconstruction of biological specimens. The proposed integrated light-sheet imaging and flow-based enquiry (iLIFE) imaging technique enables single-shot sectional imaging of a range of specimens of varying dimensions, ranging from a single cell (HeLa cell) to a multicellular organism (C. elegans). 3D reconstruction of the entire C. elegans is achieved in real-time and with an exposure time of few hundred micro-seconds. A maximum likelihood technique is developed and optimized for the iLIFE imaging system. We observed an intracellular resolution for mitochondria-labeled HeLa cells, which demonstrates the dynamic resolution of the iLIFE system. The proposed technique is a step towards achieving flow-based 3D imaging. We expect potential applications in diverse fields such as structural biology and biophysics.
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Integrated light-sheet imaging and flow-based enquiry (iLIFE) system for 3D in-vivo imaging of multicellular organism
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11 December 2017
Research Article|
December 13 2017
Integrated light-sheet imaging and flow-based enquiry (iLIFE) system for 3D in-vivo imaging of multicellular organism
Chelur K. Rasmi;
Chelur K. Rasmi
a)
1
Nanobioimaging Laboratory, IAP, Indian Institute of Science
, Bangalore 560012, India
2
Physics, Indian Institute of Science
, Bangalore 560012, India
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Sreedevi Padmanabhan;
Sreedevi Padmanabhan
3
Jawaharlal Nehru Centre for Advanced Scientific Research
, Bangalore 560064, India
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Kalyanee Shirlekar;
Kalyanee Shirlekar
4
Molecular Reproduction, Development and Genetics, Indian Institute of Science
, Bangalore 560012, India
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Kanhirodan Rajan;
Kanhirodan Rajan
2
Physics, Indian Institute of Science
, Bangalore 560012, India
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Ravi Manjithaya;
Ravi Manjithaya
3
Jawaharlal Nehru Centre for Advanced Scientific Research
, Bangalore 560064, India
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Varsha Singh;
Varsha Singh
4
Molecular Reproduction, Development and Genetics, Indian Institute of Science
, Bangalore 560012, India
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Partha Pratim Mondal
Partha Pratim Mondal
1
Nanobioimaging Laboratory, IAP, Indian Institute of Science
, Bangalore 560012, India
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a)
C. K. Rasmi and P. P. Mondal contributed equally to this work.
b)
Author to whom correspondence should be addressed: partha@iap.iisc.ernet.in.
Appl. Phys. Lett. 111, 243702 (2017)
Article history
Received:
October 20 2017
Accepted:
November 24 2017
Citation
Chelur K. Rasmi, Sreedevi Padmanabhan, Kalyanee Shirlekar, Kanhirodan Rajan, Ravi Manjithaya, Varsha Singh, Partha Pratim Mondal; Integrated light-sheet imaging and flow-based enquiry (iLIFE) system for 3D in-vivo imaging of multicellular organism. Appl. Phys. Lett. 11 December 2017; 111 (24): 243702. https://doi.org/10.1063/1.5009782
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