With great potential in precision medical application, cell biomechanics is rising as a hot topic in biology. Cell nucleus, as the largest component within cell, not only contributes greatly to the cell's mechanical behavior, but also serves as the most vital component within cell. However, cell nucleus' mechanics is still far from unambiguous up to now. In this paper, we attempted to characterize and evaluate the mechanical property of isolated cell nuclei using Atomic Force Microscopy with a tipless probe. As indicated from typical indentation, changing loading rate and stress relaxation experiment results, cell nuclei showed significant dynamically mechanical property, i.e., time-dependent mechanics. Furthermore, through theoretical analysis, finite element simulation and stress relaxation experiment, the nature of nucleus' mechanics was better described by poroelasticity, rather than viscoelasticity. Therefore, the essence of nucleus' mechanics was clarified to be poroelastic through a sophisticated analysis. Finally, we estimated the poroelastic parameters for nuclei of two types of cells through a combination of experimental data and finite element simulation.
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21 November 2016
Research Article|
November 21 2016
Poroelasticity of cell nuclei revealed through atomic force microscopy characterization
Fanan Wei;
Fanan Wei
1School of Mechanical Engineering and Automation,
Fuzhou University
, Fuzhou 350116, China
2State Key Laboratory of Robotics, Shenyang Institute of Automation,
Chinese Academy of Sciences
, Shenyang 110016, China
3
University of Chinese Academy of Sciences
, Beijing 100049, China
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Fei Lan;
Fei Lan
4Department of Electrical and Computer Engineering,
University of Pittsburgh
, Pittsburgh, Pennsylvania 15261, USA
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Bin Liu;
Bin Liu
2State Key Laboratory of Robotics, Shenyang Institute of Automation,
Chinese Academy of Sciences
, Shenyang 110016, China
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Lianqing Liu;
Lianqing Liu
a)
2State Key Laboratory of Robotics, Shenyang Institute of Automation,
Chinese Academy of Sciences
, Shenyang 110016, China
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Guangyong Li
Guangyong Li
a)
4Department of Electrical and Computer Engineering,
University of Pittsburgh
, Pittsburgh, Pennsylvania 15261, USA
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a)
Authors to whom correspondence should be addressed. Electronic addresses: [email protected], [email protected]
Appl. Phys. Lett. 109, 213701 (2016)
Article history
Received:
August 22 2016
Accepted:
November 08 2016
Citation
Fanan Wei, Fei Lan, Bin Liu, Lianqing Liu, Guangyong Li; Poroelasticity of cell nuclei revealed through atomic force microscopy characterization. Appl. Phys. Lett. 21 November 2016; 109 (21): 213701. https://doi.org/10.1063/1.4968191
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