Regeneration of a tissue fragment of freshwater polyp Hydra is accompanied by significant morphological fluctuations, suggesting the generation of active forces. In this study, we utilized a two fingered micro-robotic hand to gain insights into the mechanics of regenerating tissues. Taking advantage of a high force sensitivity (∼1 nN) of our micro-hand, we non-invasively acquired the bulk elastic modulus of tissues by keeping the strain levels low (ε < 0.15). Moreover, by keeping the strain at a constant level, we monitored the stress relaxation of the Hydra tissue and determined both viscous modulus and elastic modulus simultaneously, following a simple Maxwell model. We further investigated the correlation between the frequency of force fluctuation and that of morphological fluctuation by monitoring one “tweezed” tissue and the other “intact” tissue at the same time. The obtained results clearly indicated that the magnitude and periodicity of the changes in force and shape are directly correlated, confirming that our two fingered micro-hand can precisely quantify the mechanics of soft, dynamic tissue during the regeneration and development in a non-invasive manner.
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7 March 2016
Research Article|
March 11 2016
Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand
M. Veschgini;
M. Veschgini
a)
1Institute for Integrated Cell-Material Sciences (WPI iCeMS),
Kyoto University
, 606-8501 Kyoto, Japan
2Physical Chemistry of Biosystems, Institute of Physical Chemistry,
University of Heidelberg
, D69120 Heidelberg, Germany
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F. Gebert;
F. Gebert
a)
1Institute for Integrated Cell-Material Sciences (WPI iCeMS),
Kyoto University
, 606-8501 Kyoto, Japan
2Physical Chemistry of Biosystems, Institute of Physical Chemistry,
University of Heidelberg
, D69120 Heidelberg, Germany
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N. Khangai;
N. Khangai
a)
3Division of Systems Science, Department of Systems Innovation, Graduate School of Engineering Science,
Osaka University
, 560-8531 Toyonaka, Japan
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H. Ito;
H. Ito
4Department of Physics, Graduate School of Science,
Kyoto University
, 606-8501 Kyoto, Japan
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R. Suzuki;
R. Suzuki
1Institute for Integrated Cell-Material Sciences (WPI iCeMS),
Kyoto University
, 606-8501 Kyoto, Japan
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T. W. Holstein;
T. W. Holstein
5Molecular Evolution and Genomics, Centre for Organismal Studies (COS),
University of Heidelberg
, D69120 Heidelberg, Germany
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Y. Mae;
Y. Mae
3Division of Systems Science, Department of Systems Innovation, Graduate School of Engineering Science,
Osaka University
, 560-8531 Toyonaka, Japan
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T. Arai;
T. Arai
b)
3Division of Systems Science, Department of Systems Innovation, Graduate School of Engineering Science,
Osaka University
, 560-8531 Toyonaka, Japan
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a)
M. Veschgini, F. Gebert, and N. Khangai contributed equally to this work.
b)
Authors to whom correspondence should be addressed. Electronic addresses: arai@sys.es.osaka-u.ac.jp and tanaka@uni-heidelberg.de.
Appl. Phys. Lett. 108, 103702 (2016)
Article history
Received:
December 16 2015
Accepted:
February 24 2016
Citation
M. Veschgini, F. Gebert, N. Khangai, H. Ito, R. Suzuki, T. W. Holstein, Y. Mae, T. Arai, M. Tanaka; Tracking mechanical and morphological dynamics of regenerating Hydra tissue fragments using a two fingered micro-robotic hand. Appl. Phys. Lett. 7 March 2016; 108 (10): 103702. https://doi.org/10.1063/1.4943402
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