Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.
Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation
I. V. Fedotov, L. V. Doronina-Amitonova, D. A. Sidorov-Biryukov, A. B. Fedotov, K. V. Anokhin, S. Ya. Kilin, K. Sakoda, A. M. Zheltikov; Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation. Appl. Phys. Lett. 24 February 2014; 104 (8): 083702. https://doi.org/10.1063/1.4864646
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