Cell lysis is a key sample preparation stage in many biomedical studies as DNA extraction and classification require the use of the nucleic acid and proteins released upon decomposition of a cell membrane. We present an effective method of lysing cells suspended in a microliter droplet placed on a super-hydrophobic surface. When a bubble, injected into the sessile droplet, subsequently ruptures, a rapidly moving fluid jet is formed. In this work, cells that are transported within this fluid jet are captured on a separate hydrophilic substrate and are shown to have been lysed.
REFERENCES
1.
M.
Mahalanabis
, H.
Al-Muayad
, M.
Dominika Kulinski
, D.
Altman
, and C. M.
Klapperich
, Lab Chip
9
(19
), 2811
(2009
).2.
A.
Salonen
, J.
Nikkilä
, J.
Jalanka-Tuovinen
, O.
Immonen
, M.
Rajilić-Stojanović
, R. A.
Kekkonen
, A.
Palva
, and W. M.
de Vos
, J. Microbiol. Methods
81
(2
), 127
(2010
).3.
M. Q.
Thanh Tran
, Y.
Nygren
, C.
Lundin
, P.
Naredi
, and E.
Björn
, Anal. Biochem.
396
(1
), 76
(2010
).4.
M.
Karle
, J.
Miwa
, G.
Czilwik
, V.
Auwärter
, G.
Roth
, R.
Zengerle
, and F.
von Stetten
, Lab Chip
10
(23
), 3284
(2010
).5.
A.
Schmid
, H.
Kortmann
, P. S.
Dittrich
, and L. M.
Blank
, Curr. Opin. Biotechnol.
21
(1
), 12
(2010
).6.
G.
Velve-Casquillas
, M.
Le Berre
, M.
Piel
, and P. T.
Tran
, Nano Today
5
(1
), 28
(2010
).7.
M. L.
Kovarik
, P. C.
Gach
, D. M.
Ornoff
, Y.
Wang
, J.
Balowski
, L.
Farrag
, and N. L.
Allbritton
, Anal. Chem.
84
(2
), 516
(2012
).8.
K. A.
Sookraj
, W. B.
Bowne
, V.
Adler
, E.
Sarafraz-Yazdi
, J.
Michl
, and M. R.
Pincus
, Cancer Chemother. Pharmacol.
66
(2
), 325
(2010
).9.
L.
Liu-Yesucevitz
, A.
Bilgutay
, Y.-J.
Zhang
, T.
Vanderwyde
, A.
Citro
, T.
Mehta
, N.
Zaarur
, A.
McKee
, R.
Bowser
, and M.
Sherman
, PLoS One
5
(10
), e13250
(2010
).10.
L.
Kocgozlu
, M.
Rabineau
, G.
Koenig
, Y.
Haikel
, P.
Schaaf
, J.-N.
Freund
, J.-C.
Voegel
, P.
Lavalle
, and D.
Vautier
, Biomaterials
33
(3
), 798
(2012
).11.
R. B.
Brown
and J.
Audet
, J. R. Soc. Interface
5
(Suppl 2
), S131
(2008
).12.
J.
Kim
, M.
Johnson
, P.
Hill
, and B. K.
Gale
, Integr. Biol.
1
(10
), 574
(2009
).13.
R. N.
Zare
and S.
Kim
, Annu. Rev. Biomed. Eng.
12
, 187
(2010
).14.
N.
Privorotskaya
, Y.-S.
Liu
, J.
Lee
, H.
Zeng
, J. A.
Carlisle
, A.
Radadia
, L.
Millet
, R.
Bashir
, and W. P.
King
, Lab Chip
10
(9
), 1135
(2010
).15.
G.
Mernier
, N.
Piacentini
, T.
Braschler
, N.
Demierre
, and P.
Renaud
, Lab Chip
10
(16
), 2077
(2010
).16.
F.
Nederberg
, Y.
Zhang
, J. P. K.
Tan
, K.
Xu
, H.
Wang
, C.
Yang
, S.
Gao
, X. D.
Guo
, K.
Fukushima
, and L.
Li
, Nat. Chem.
3
(5
), 409
(2011
).17.
D.
Di Carlo
, K.-H.
Jeong
, and L. P.
Lee
, Lab Chip
3
(4
), 287
(2003
).18.
P. E.
Vandeventer
, K. M.
Weigel
, J.
Salazar
, B.
Erwin
, B.
Irvine
, R.
Doebler
, A.
Nadim
, G. A.
Cangelosi
, and A.
Niemz
, J. Clin. Microbiol.
49
(7
), 2533
(2011
).19.
Y.
Zhou
, K.
Yang
, J.
Cui
, J. Y.
Ye
, and C. X.
Deng
, J. Controlled Rel.
157
(1
), 103
(2012
).20.
Z. G.
Li
, A. Q.
Liu
, E.
Klaseboer
, J. B.
Zhang
, and C. D.
Ohl
, Lab Chip
13
(6
), 1144
(2013
).21.
H.
Hoefemann
, S.
Wadle
, N.
Bakhtina
, V.
Kondrashov
, N.
Wangler
, and R.
Zengerle
, Sens. Actuators, B
168
, 442
(2012
).22.
J. N.
Tan
, T.
Alan
, and A.
Neild
, AIP Adv.
3
(2
), 022121
(2013
).23.
U.-C.
Yi
and C.-J.
Kim
, Sens. Actuators, A
114
(2
), 347
(2004
).24.
U.-C.
Yi
and C.-J.
Kim
, Mechanical and Aerospace Engineering Department
, University of California
, Los Angeles (UCLA)
, 2004
.25.
J. N.
Tan
, W. Y. L.
Ling
, and A.
Neild
, Appl. Phys. Express
6
(7
), 077301
(2013
).26.
I. A.
Larmour
, S. E.
Bell
, and G. C.
Saunders
, Angew. Chem., Int. Ed.
46
(10
), 1710
(2007
);27.
28.
J. M.
Bergthorson
, K.
Sone
, T. W.
Mattner
, P. E.
Dimotakis
, D. G.
Goodwin
, and D. I.
Meiron
, Phys. Rev. E
72
(6
), 066307
(2005
).29.
P.
Marmottant
and S.
Hilgenfeldt
, Nature
423
(6936
), 153
(2003
).© 2014 AIP Publishing LLC.
2014
AIP Publishing LLC
You do not currently have access to this content.
