In this work, a micro-fluxgate-based biosensor was designed for the detection of carcinoembryonic antigen (CEA) labeled by Dynabeads. The sensor with Fe-based amorphous core and three dimension solenoid coils was fabricated by Micro Electro-Mechanical system technology. Sandwich assays are performed using antibody-antigen pair combination of biotin-streptavidin assay on a separated Au film substrate surface with a self-assembled layer. With dc magnetic fields in the range of 560 μT to 875 μT, detection of CEAs with different concentrations was performed and a minimum detectable concentration of 1 pg/ml was achieved. Furthermore, CEA samples with different concentrations can be distinguished.
REFERENCES
1.
J.
Porter
, J.
Robinson
, R.
Pickup
, and C.
Edwards
, J. Appl. Microbiol.
84
, 722
–732
(1998
).2.
J.
Oster
, J.
Parker
, and L.
a Brassard
, J. Magn. Magn. Mater.
225
, 145
–150
(2001
).3.
T. N.
Krogh
, T.
Berg
, and P.
Højrup
, Anal. Biochem.
274
, 153
–162
(1999
).4.
B.-H.
Jun
, M. S.
Noh
, G.
Kim
, H.
Kang
, J.-H.
Kim
, W.-J.
Chung
, M.-S.
Kim
, Y.-K.
Kim
, M.-H.
Cho
, D. H.
Jeong
, and Y.-S.
Lee
, Anal. Biochem.
391
, 24
–30
(2009
).5.
T.
Aytur
, J.
Foley
, M.
Anwar
, B.
Boser
, E.
Harris
, and P. R.
Beatty
, J. Immunol. Methods
314
, 21
–29
(2006
).6.
M. L. H.
Gruwel
, Y.
Yang
, P.
de Gervai
, J.
Sun
, and V. V.
Kupriyanov
, Magn. Reson. Imaging
27
, 970
–977
(2009
).7.
X. J. A.
Janssen
, A. J.
Schellekens
, K.
van Ommering
, L. J.
van IJzendoorn
, and M. W. J.
Prins
, Biosens. Bioelectron.
24
, 1937
–1941
(2009
).8.
M. J.
Duffy
, A.
van Dalen
, C.
Haglund
, L.
Hansson
, R.
Klapdor
, R.
Lamerz
, O.
Nilsson
, C.
Sturgeon
, and O.
Topolcan
, Eur. J. Cancer
39
, 718
–727
(2003
).9.
H.
Chon
, S.
Lee
, S. W.
Son
, C. H.
Oh
, and J.
Choo
, Anal. Chem.
81
, 3029
–3034
(2009
).10.
J.
Han
, Y.
Zhuo
, Y. Q.
Chai
, L.
Mao
, Y. L.
Yuan
, and R.
Yuan
, Talanta
85
, 130
–135
(2011
).11.
M. Y.
Liu
, C. P.
Jia
, Q. H.
Jin
, X. H.
Yao
, J. Q.
Xiang
, and J. L.
Zhao
, Talanta
81
, 1625
–1629
(2010
).12.
D. M. P.
Thomson
, J.
Krupey
, S. O.
Freedman
, and P.
Gold
, Proc. Natl. Acad. Sci.
64
, 161
–167
(1969
).13.
J. H.
Lin
, F.
Yan
, and H. X.
Ju
, Clin. Chim. Acta
341
, 109
–115
(2004
).14.
F.
Yan
, J. N.
Zhou
, J. H.
Lin
, H. X.
Ju
, and X. Y.
Hu
, J. Immunol. Methods
305
, 120
–127
(2005
).15.
Z.
Dai
, F.
Yan
, H.
Yu
, X. Y.
Hu
, and H. X.
Ju
, J. Immunol. Methods
287
, 13
–20
(2004
).16.
J.
Wu
, J. H.
Tang
, Z.
Dai
, F.
Yan
, H. X.
Ju
, and N. E.
Murr
, Biosens. Bioelectron.
22
, 102
–108
(2006
).17.
F.
Tan
, F.
Yan
, and H. X.
Ju
, Electrochem. Commun.
8
, 1835
–1839
(2006
).18.
G.
Kurlyandskaya
and V.
Levit
, Biosens. Bioelectron.
20
, 1611
–1616
(2005
).19.
P.-A.
Besse
, G.
Boero
, M.
Demierre
, V.
Pott
, and R.
Popovic
, Appl. Phys. Lett.
80
, 4199
–4201
(2002
).20.
H. A.
Ferreira
, D. L.
Graham
, P. P.
Freitas
, and J. M. S.
Cabral
, J. Appl. Phys.
93
, 7281
–7286
(2003
).21.
T. M.
Liakopoulos
and C. H.
Ahn
, Sens. Actuator A
77
, 66
–72
(1999
).22.
F.
Primdahl
, J. Phys. E
12
, 241
–253
(1979
).23.
J.
Lenz
, Proc. IEEE
78
, 973
–989
(1990
).24.
P.
Ripka
, J. Magn. Magn. Mater.
215–216
, 735
–739
(2000
).25.
J. V.
Jokerst
, A.
Raamanathan
, N.
Christodoulides
, P. N.
Floriano
, A. A.
Pollard
, G. W.
Simmons
, J.
Wong
, C.
Gage
, W. B.
Furmaga
, S. W.
Redding
, and J. T.
McDevitt
, Biosens. Bioelectron.
24
, 3622
–3629
(2009
).26.
K.
Sato
, M.
Tokeshi
, H.
Kimura
, and T.
Kitamori
, Anal. Chem.
73
, 1213
–1218
(2001
).© 2013 AIP Publishing LLC.
2013
AIP Publishing LLC
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