We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli β-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 105 droplets per second, an analytical throughput larger than those obtained using flow cytometry.
Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array
Jiseok Lim, Jérémy Vrignon, Philipp Gruner, Christos S. Karamitros, Manfred Konrad, Jean-Christophe Baret; Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array. Appl. Phys. Lett. 11 November 2013; 103 (20): 203704. https://doi.org/10.1063/1.4830046
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