Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique–fluorescence-assisted photoacoustic thermometry (FAPT)–for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.
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Research Article| May 16 2013
Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry
Liang Gao, Chi Zhang, Chiye Li, Lihong V. Wang; Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry. Appl. Phys. Lett. 13 May 2013; 102 (19): 193705. https://doi.org/10.1063/1.4807140
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