Direct microscopic fluorescence imaging of single molecules can provide a wealth of mechanistic information, but up to now, it has not been possible under high pressure conditions, due to limitations in microscope pressure cell design. We describe a pressure cell window design that makes it possible to image directly single molecules at high hydrostatic pressure. We demonstrate our design by imaging single molecules of Alexa Fluor 647 dye bound to DNA, at 120 and 210 bar, and following their fluorescence photodynamics. We further show that the failure pressure of this type of pressure cell window can be in excess of 1 kbar.
Single-molecule imaging at high hydrostatic pressure
Hugh Vass, S. Lucas Black, Cristina Flors, Diarmuid Lloyd, F. Bruce Ward, Rosalind J. Allen; Single-molecule imaging at high hydrostatic pressure. Appl. Phys. Lett. 15 April 2013; 102 (15): 154103. https://doi.org/10.1063/1.4802202
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