In this study, C-scan based high resolution photoacoustic microscopy (CPAM) is devised and used to invivo image the DsRed-expressing cells in the central nervous system (CNS) of Drosophila pupae. The quality of out-of-focus images was improved significantly from both the phantom and invivo experiments. Organs containing DsRed-expressing cells in Drosophila pupae can be imaged using our CPAM and agreed well with the histological sections. This technology allows rapid imaging and monitoring of organismal development in mesoscopic-scale animals as well as the generation of a detailed atlas of CNS development in the pupal stage.
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