We report an electrochemically synthesized ZnS nanocrystals modified polypyrrole (PPy) nanocomposite film based immunosensor for the detection of C-reactive protein (αCRP). The ZnS-PPy composite film was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemical techniques. The modified film showed good biocompatibility with efficient binding to protein antibody (αCRP-Ab) molecules through ZnS nanocrystals, exhibited an attractive platform for immunosensor fabrication. The electrical and sensing properties of the polymer composite film of different thickness towards protein antigen (αCRP-Ag) were delineated. The immunosensor exhibited an impedance response to αCRP-Ag concentration in a linear range from 10 ng to 10 μg mL−1.

Topics
Atomic force microscopy, Polymers, Biomaterials, Thin films, Transmission electron microscopy, Nanocomposites, Nanocrystals, Antibody, Antigen, Proteins
1.
Y.
 
Li
,
A.
 
Rizzo
,
R.
 
Cingolani
, and
G.
 
Gigli
,
Adv. Mater.
 
18
,
2545
(
2006
).
https://doi.org/10.1002/adma.200600181
Google Scholar
Crossref
Search ADS
 
2.
Q.
 
Wang
 and
D. K.
 
Seo
,
Chem. Mater.
 
18
,
5764
(
2006
).
https://doi.org/10.1021/cm061051j
Google Scholar
Crossref
Search ADS
 
3.
S.
 
Coe
,
W. K.
 
Woo
,
M. G.
 
Bawendi
, and
V.
 
Bulovic
,
Nature
 
420
,
800
(
2002
).
https://doi.org/10.1038/nature01217
Google Scholar
Crossref
Search ADS
PubMed
 
4.
W.
 
Jiang
,
S.
 
Mardyani
,
H.
 
Fischer
, and
C. W.
 
Chan
,
Chem. Mater.
 
18
,
872
(
2006
).
https://doi.org/10.1021/cm051393+
Google Scholar
Crossref
Search ADS
 
5.
Y.
 
Zheng
,
S.
 
Gao
, and
J. Y.
 
Ying
,
Adv. Mater.
 
19
,
376
(
2007
).
https://doi.org/10.1002/adma.200600342
Google Scholar
Crossref
Search ADS
 
6.
Rajesh
,
Basanta K.
 
Das
,
Sira
 
Srinives
, and
Ashok
 
Mulchandani
,
Appl. Phys. Lett.
 
98
,
013701
(
2011
).
https://doi.org/10.1063/1.3529951
Google Scholar
Crossref
Search ADS
PubMed
 
7.
Lina
 
Wu
,
Mike
 
McIntosh
,
Xueji
 
Zhang
, and
Huangxian
 
Ju
,
Talanta
 
74
,
387
(
2007
).
https://doi.org/10.1016/j.talanta.2007.09.023
Google Scholar
Crossref
Search ADS
PubMed
 
8.
Ya-Ping
 
Hsiao
,
Wan-Yu
 
Su
,
Jin-Ru
 
Cheng
, and
Shu-Hua
 
Cheng
,
Electrochim. Acta
 
56
,
6887
(
2011
).
https://doi.org/10.1016/j.electacta.2011.06.031
Google Scholar
Crossref
Search ADS
 
9.
C. M.
 
Li
,
C. Q.
 
Sun
,
S.
 
Song
,
V. E.
 
Choong
,
G.
 
Maracas
, and
X. J.
 
Zhang
,
Front. Biosci.
 
10
,
180
(
2005
).
https://doi.org/10.2741/1519
Google Scholar
Crossref
Search ADS
PubMed
 
10.
A.
 
Kaushik
,
P. R.
 
Solanki
,
M. K.
 
Pandey
,
S.
 
Ahmad
, and
B. D.
 
Malhotra
,
Appl. Phys. Lett.
 
95
,
173703
(
2009
).
https://doi.org/10.1063/1.3249586
Google Scholar
Crossref
Search ADS
 
11.
Alan S.
 
Maisel
,
Vikas
 
Bhalla
, and
Eugene
 
Braunwald
,
Nature Rev. Cardiol.
 
3
,
24
(
2006
).
Google Scholar
Crossref
Search ADS
 
12.
A.
 
Dolci
 and
M.
 
Panteghini
,
Clinica Chimica Acta
 
369
,
179
(
2006
).
https://doi.org/10.1016/j.cca.2006.02.042
Google Scholar
Crossref
Search ADS
 
13.
Rajesh
,
Vikash
 
Sharma
,
Vinod
 
Kumar Tanwar
, and
A. M.
 
Biradar
,
Sens. Lett.
 
8
,
362
(
2010
).
https://doi.org/10.1166/sl.2010.1278
Google Scholar
Crossref
Search ADS
 
14.
K.
 
Kriz
,
F.
 
Ibraimi
,
M.
 
Lu
,
L. O.
 
Hansson
, and
D.
 
Kriz
,
Anal. Chem.
 
77
,
5920
(
2005
).
https://doi.org/10.1021/ac0508649
Google Scholar
Crossref
Search ADS
PubMed
 
15.
J.
 
Ramo´n-Azco´n
,
E.
 
Valera
, and
Á.
 
Rodŕιguez
,
Biosens. Bioelec.
 
23
,
1367
(
2008
).
https://doi.org/10.1016/j.bios.2007.12.010
Google Scholar
Crossref
Search ADS
 
16.
M.
 
Hnaien
,
M. F.
 
Diouani
, and
S.
 
Helali
,
Biochem. Eng. J.
 
39
,
443
(
2008
).
https://doi.org/10.1016/j.bej.2007.09.018
Google Scholar
Crossref
Search ADS
 
17.
Y. P.
 
Timalsina
,
J.
 
Branan
,
B. E.
 
Aston
,
K.
 
Noren
,
G.
 
Corti
,
R.
 
Schumacher
, and
D. N.
 
Ncllroy
,
J. Appl. Phys.
 
110
,
014901
(
2011
).
https://doi.org/10.1063/1.3601521
Google Scholar
Crossref
Search ADS
 
18.
Rajesh
,
T.
 
Sarkar
, and
A.
 
Mulchandani
,
Appl. Phys. Lett.
 
99
,
173110
(
2011
).
https://doi.org/10.1063/1.3656283
Google Scholar
Crossref
Search ADS
 
19.
M. K.
 
Ram
,
M.
 
Adami
,
S.
 
Paddeu
, and
C.
 
Nicolini
,
Nanotechnology
 
11
,
112
(
2000
).
https://doi.org/10.1088/0957-4484/11/2/312
Google Scholar
Crossref
Search ADS
 
20.
A.
 
Joshi
,
D. K.
 
Aswal
,
S. K.
 
Gupta
,
J. V.
 
Yakhmi
, and
S. A.
 
Gangal
,
Appl. Phys. Lett.
 
94
,
103115
(
2009
).
https://doi.org/10.1063/1.3093499
Google Scholar
Crossref
Search ADS
 
21.
R. G.
 
Davidson
 and
T. G.
 
Turner
,
Synth. Met.
 
72
,
121
(
1995
).
https://doi.org/10.1016/0379-6779(94)02332-S
Google Scholar
Crossref
Search ADS
 
22.
M.
 
Gamero
,
F.
 
Pariente
,
E.
 
Lorenzo
, and
C.
 
Alonso
,
Biosens. Bioelectron.
 
25
,
2038
(
2010
).
https://doi.org/10.1016/j.bios.2010.01.032
Google Scholar
Crossref
Search ADS
PubMed
 
23.
F.
 
Lisdat
 and
D.
 
Schafer
,
Anal. Bioanal. Chem.
 
391
,
1555
(
2008
).
https://doi.org/10.1007/s00216-008-1970-7
Google Scholar
Crossref
Search ADS
PubMed
 
24.
B.
 
McDonnell
,
S.
 
Hearty
,
P.
 
Leonard
, and
R.
 
O’Kennedy
,
Clin. Biochem.
 
42
,
549
(
2009
).
https://doi.org/10.1016/j.clinbiochem.2009.01.019
Google Scholar
Crossref
Search ADS
PubMed
 
25.
See supplementary material at http://dx.doi.org/10.1063/1.3681580 for detail device fabrication with scheme and CV figures and Table I.
© 2012 American Institute of Physics.
2012
American Institute of Physics

Supplementary Material

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