Lipase is an industrially important enzyme and is usually obtained from microorganisms, especially filamentous fungi, producing it in a high amount. The method commonly used to produce lipase from filamentous fungi is Submerged Fermentation (SmF) with agitation. However, agitation requires energy and high production cost. Hence, reducing the use of agitation is needed. This research aims to observe the influence of agitation speed toward colony growth, lipase productivity, and nutrient absorption from Indonesian Aspergillus aculeatus Ms.11 in broth medium. Aspergillus aculeatus Ms.11 was inoculated into 50 mL fermentation medium, which contains 1% of olive oil and glucose mixture with 1:1 comparison. Incubation was done using varied agitation speed (0, 50, 100, 150, and 200 rpm) at 30 °C for 96 h. Relative activity lipase productivity, enzyme-specific activity, and glucose and nitrogen consumption were determined after the incubation. The results show that the highest lipase activity (6.33 U/mL) and lipase specific activity (0.10 U/mg) are observed at 0 rpm, while the highest lipase productivity (0.43 U/mg) is observed at 50 rpm. We concluded that maximum lipase production from Ms. 11 can be obtained using low agitation speed. Further study to optimize carbon and nitrogen sources is suggested.

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