Diabetes Mellitus (DM) and COVID-19 diseases are still global problems. One of the causes of DM, mainly type 2 is unhealthy eating habits, including white sugar, which contains almost no active compounds except sucrose. The active compounds in sugarcane juice such as ferulic acid, gallic acid, 4-Hydroxy benzoate acid, and vanillic acid, which are suspected besides alpha-amylase inhibitors, also have potential as anti COVID-19. The purpose of this research is to extract secondary metabolites from sugarcane juice, determine TPC and TFC, and to know in silico activity of the active compounds in sugarcane juice as an alpha-amylase inhibitor and binding position to the active site of the spike protein. The results have shown that TPC and TFC in sugarcane juice are 0.5501% and 0.4480%. The in silico analysis has proven that the ferulic acid compound is bonded to the alpha-amylase binding site as acarbose did. Gallic acid, 4-hydroxy benzoic acid, and vanillic acid compounds are bonded to RBD of S protein COVID-19 as chloroquine did, with various binding affinity. These results indicate that phenolic and flavonoids in sugarcane juice have the potential as an alpha-amylase inhibitor and anti COVID-19.

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