Phytochemical and antioxidant activities of Robusta coffee leaves extracts from Alor Island, East Nusa Tenggara

Coffee is a very promising commodity. Besides being consumed as a famous drink with high caffeine content, coffee also has many compounds that can act as antioxidants. One part of the coffee plant that acts as an antioxidant is the leaf. Robusta coffee (Coffea canephora) is one species of the genus Coffee. The aims of this study were to determined the phytochemical and antioxidant activity on Robusta coffee leaves as seen based on differences in the ecology of the highlands on Alor Island – East Nusa Tenggara. Robusta coffee leaves were extracted by maceration for 24 hours with methanol. DPPH assay was used to determine the antioxidant activity. The result showed that Robusta coffee leaves from Alor Island give the antioxidant activity with IC₅₀ 56,337 ppm. Phytochemical analysis revealed that the bioactive compound from Robusta coffee leaves is composed of alkaloids, flavonoids, terpenoids, and phenolic.