The herbal plants commonly used in Indonesia are Jati belanda (Guazuma ulmifolia L) which is commonly used as a medicine for high blood pressure and cancer. During the storage, to reduce contamination from bacteria, fungi, and other microorganisms can be used gamma irradiation. Gamma Irradiation Co-60 can be used as an alternative for preserving herbal plants. This study aimed to examine the effect of Co-60 (0 and 7.5 kGy) gamma-irradiation on G. ulmifolia plants on anti-cancer line properties (THP-1, K-562, and MCF-7). G.ulmifolia herb without irradiation (0 kGy) and gamma-irradiated 7.5 kGy using Co-60. The samples were macerated using ethanol as much as 2 replications. Cytotoxic tests were performed on ethanol extracts against line cancer cells (THP-1, K-562, and MCF-7) with varying concentrations of 5, 10, 20, 40, and 80 µg / ml. The results showed that irradiation doses of 0 kGy and 7.5 kGy were very active in inhibiting K-562 cancer cells with IC50 respectively 10.94 µg/ml and 16.74 µg/ml. Gamma irradiation can be used as an alternative in preserving medicinal plants without reducing the anti-cancer properties from the G.ulmifolia.

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