Cytotoxic effects and apoptosis of solo black garlic (Allium sativum L.) extract on T47D breast cancer cell line

Breast cancer frequently occurs to woman and at least there are 1.67 million cases of breast cancer in 2012. One of alternative medications which is relatively cheap and without side effect is the use of single black garlic. The activity of anti-cancer on single black garlic can pass the apoptosis path which causes cancer cell to be dead and anti- proliferation path which causes the growth of cancer cell to be inhibited. This research aims to study the effect solo black garlic extract (hexane, chloroform, ethyl acetate) towards the breast cancer cell T47D. The method used in this research was extraction, freeze drying to prepare the sample, MTT Assay to examine the cytotoxicity and also Flow-cytometry to the test of apoptosis cell T47D. The result of the cytocytoxity test known that there kinds of extract which have potency to decrease the viability percentage of cell T47D which is more than 50% in the concentration less than 2000 µg/ml, that is single black garlic's hexane as much 1.558 µg/ml, chloroform as much 658µl, and extract of ethyl acetate of single black garlic as much 551 µg/ml. The result of apoptosis test shows that the extract of single black garlic has potency towards the induction of apoptosis cell T47D.