The cow releases feces about 10 kilograms per day. Cow feces will still become garbage or only become manure if not used as biogas material. Biogas is a gas produced by anaerobic activity or fermentation from organic materials, such as cow feces. The main content of biogas is methane gas. The by-product of biogas is biogas sludge. Sludge biogas is the mud that comes out of the biogas reactor outlet section this mud still contains nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg), calcium (Ca) and iron (Fe). Rice is the main food commodity plant in Indonesia. Segreng is variety of rice which one of the Gunungkidul Regency germplasm. The purpose of this study was to analyze the growth of Segreng rice which was given the treatment of biogas sludge and determining the optimum dose of sludge biogas fertilizer in Segreng rice plant. The study was conducted in 4 treatments, namely doses of 0 ml (control), 4 ml, 8 ml, 12 ml and 24 ml sludge biogas every polybag containing one individual plant. Polybag contains 5 kg soil. The parameters measured were plant height, leaf number, number of tillers and leaf chlorophyll content. The research design used was a Completely Randomized Design (CRD). The results obtained were tested by One Way ANOVA followed by DMRT Test with a confidence level of 95% (α=0.05). The results showed the highest plant height and number of tiller in rice plant treated with 4 ml sludge biogas. While the highest chlorophyll content and leaves number were obtained in control plants. Then the optimum dose for plant height and number of tiller is 4 ml per polybag.

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