This study aimed to find the optimum mixture of temulawak (Curcuma xanthorrhiza) and meniran (Phyllanthus niruri) extract as an antiacne based on its activity as an antibacterial against Staphylococcus epidermidis and antioxidants. Temulawak rhizome and meniran were extracted with 96% ethanol. Furthermore, simplex centroid design was used to prepared formula from extract. The tenth formula obtained and tested for their antioxidant and antibacterial activities. Formula 5 (50% temulawak and 50% meniran) as the most active antiacne formula with IC50 of antioxidant values of 9.47 ppm, the minimum inhibitory concentration (MIC) against S. epidermidis was 0.25 mg/mL, and minimum bactericidal concentration (MBC) was 1.00 mg/mL. The characteristic by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) showed that formula 5 consisted of xanthorrhizol, phyllanthin, and hypophyllanthin.

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