The effect of extract concentration of Diospyros discolor Willd. as silver nanoparticles synthesis

Diospyros discolor Willd. (Bisbul) is known to have an activity as a reducing agent to reduce silver ions into silver nanoparticles. These abilities affected by the phytochemical content in the extract, such as phenolics and flavonoids compounds, which makes the synthesis process to be environmentally friendly. In this study, various concentrations of crude water extract from Bisbul leaves were used at concentration 0.01, 0.025, and 0.05 % (w/v), with the ratio between the extract and 1 mM AgNO₃ was 1:9 (v/v). The formation of silver nanoparticles are confirmed by the presence of absorption spectra between 350-450 nm using UV-Vis spectrophotometer. Based on the results of phytochemical tests, it showed that the extract contains phenols and flavonoids compound. Furthermore, the total flavonoid content (TFC) and total phenolic content (TPC) were measured to know their presence in the extract. The amount of those compounds in each extract concentration tend to increase with the increasing of extract concentration. The extract with concentration 0.05 % had a narrower peak width based on the Full Width at Half Maximum (FWHM) value, which can indicate the monodispersity in silver nanoparticles size and distribution.