Shrimp pond waste (Litopenaeus vannamei) is a waste metabolite that produces changes in the quality of the aquatic environment and affects the abundance and composition of macrozoobenthos. This study aims to determine the impact of shrimp pond waste inputs on macrozoobenthos diversity. This quantitative descriptive study] uses a purposive sampling technique. Sampling was carried out at five stations along with the Kali Jeruk river flow with three repetitions. Macrozoobenthos identification was carried out at the Fisheries Laboratory and water testing at Perum Jasa Tirta Malang. The diversity of macrozoobenthos was analyzed using PCA tests. The results showed that the parameters that had the most direct correlation with the variety of macrozoobenthos were DO. The results of the macrozoobenthos study found ten species consisting of three classes, seven families, and nine genera. The highest macrozoobenthos diversity value at station 5 was 2.10, and the lowest at station 2 was 0.99. Shrimp pond waste has a different effect on macrozoobenthos diversity in five stations.

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