GnRH is widely used in the embryo fertilization program (FIV) as one of the ovarian stimulation regimens. At the implantation window, GnRH agonists are known to have an effect on the endometrium directly or indirectly. GnRH estimated has a negative effect on the development of endometrial cells after ovarian stimulation. This study is to analyze the impact of GnRH agonist on ovarian stimulation procedures on receptor expression and endometrial cell apoptosis. The study sample was a stored biological material (BBT) from serum and the endometrial tissue of Macaca nemestrina. The total sample is 8 and divided into 2 groups, the stimulated and control groups. Each sample will be examined 2 types which are the enzyme-linked immunosorbent assay (ELISA) for serum and immunohistochemistry (IHC) for endometrial tissue. IHC was performed with anti-GnRH and caspase 3 antibody. Concentration is measured using an ELISA reader and then converts Optical Density (OD) to a concentration using SoftMax Pro Software. Calculations of stained and unmarked cells were performed using the Image-J Analyzer programs and scored by HSCORE. There is no significant difference between GnRH serum in stimulation or control group (p=0.773). There was no correlation between GnRH in serum with GnRH receptor in endometrial tissue (p=0.060) and activation of caspase (p=0.193). There was no difference between stimulation group and control group in the amount of GnRH in serum, presence of active GnRHR, and Caspase 3 activation. Keywords: GnRH receptors, Macaca nemestria, GnRH Agonist, Controlled ovarian stimulation
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10 December 2019
THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019
22–24 July 2019
Padang, Indonesia
Research Article|
December 10 2019
GnRH receptor expression and endometrial cell proliferation of Macaca nemestrina after administration of GnRH agonist in controlled ovarian stimulation procedures
Andhea Pradhita;
Andhea Pradhita
a)
1
Master Program Biomedical sciences, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Central Jakarta 10430, Indonesia
a)Corresponding author: [email protected]
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Nurhuda Sahar;
Nurhuda Sahar
b)
2
Department of Biology, Biomedical Sciences, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Central Jakarta 10430 Indonesia
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Muharram Natadisastra;
Muharram Natadisastra
3
Department Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Central Jakarta 10430 Indonesia
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Wa Ode Zulhulaifah;
Wa Ode Zulhulaifah
1
Master Program Biomedical sciences, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Central Jakarta 10430, Indonesia
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Rosalina Thuffi
Rosalina Thuffi
1
Master Program Biomedical sciences, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Central Jakarta 10430, Indonesia
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2193, 040006 (2019)
Citation
Andhea Pradhita, Nurhuda Sahar, Muharram Natadisastra, Wa Ode Zulhulaifah, Rosalina Thuffi; GnRH receptor expression and endometrial cell proliferation of Macaca nemestrina after administration of GnRH agonist in controlled ovarian stimulation procedures. AIP Conf. Proc. 10 December 2019; 2193 (1): 040006. https://doi.org/10.1063/1.5139368
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