Previous studies have shown that alpha-mangostin has cytotoxic potentials by increasing apoptotic and promoting arrest in the cell cycle process in hepatocellular carcinoma in vitro. Prior research has also shown that alpha- mangostin ameliorates alcohol’s toxicity in hepatic stellate cells in vitro; however, whether alpha-mangostin exerts its effect by inducing apoptosis is still unknown. The study was aimed to analyze the apoptotic properties of alpha-mangostin in acetaldehyde-induced hepatic stellate cells in vitro. Immortalized human hepatic stellate (HSC) cells with LX2 cell line were treated with acetaldehyde in the presence or absence of alpha-mangostin (10 and 20 µM). After treatments, cells were harvested, isolated for RNA, and analyzed for mRNA expressions of apoptotic markers: Bax, Bcl-2, caspase-3, and caspase-9 using qRT-PCR. Acetaldehyde treatment caused a significant reduction in the mRNA expressions of Bax and caspase-3 and a slight decrease in the expressions of caspase-9. The addition of alpha-mangostin increased the mRNA expressions of Bax, caspase-9, and caspase-3 significantly. Increased mRNA expressions of Bcl-2 in alpha-mangostin- treated cells were observed. These effects displayed a dose-dependent tendency. Alpha-mangostin demonstrates apoptotic activity in acetaldehyde-induce hepatic stellate cells in a dose-dependent manner.
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10 December 2019
THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019
22–24 July 2019
Padang, Indonesia
Research Article|
December 10 2019
Apoptotic activity of alpha-mangostin in acetaldehyde- induced LX2 hepatic stellate cell lines
Novriantika Lestari;
Novriantika Lestari
1
Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia
2
Department of Pharmacology, Faculty of Medicine and Health Sciences, University of Bengkulu
, Jl. W.R Supratman, Kandang Limun, Bengkulu 38371 Indonesia
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Daniel Amartya;
Daniel Amartya
3
Medical Student, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia
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Vivian Soetikno;
Vivian Soetikno
4
Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia
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Melva Louisa
Melva Louisa
a)
4
Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia
, Jl Salemba Raya No 6, Senen, Central Jakarta 10430 Indonesia
a)Corresponding author: [email protected]
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a)Corresponding author: [email protected]
AIP Conf. Proc. 2193, 030019 (2019)
Citation
Novriantika Lestari, Daniel Amartya, Vivian Soetikno, Melva Louisa; Apoptotic activity of alpha-mangostin in acetaldehyde- induced LX2 hepatic stellate cell lines. AIP Conf. Proc. 10 December 2019; 2193 (1): 030019. https://doi.org/10.1063/1.5139356
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