Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.

1.
Dengue and severe dengue [Internet]
.
Who.int.
2018
. Available from: http://www.who.int/mediacentre/factsheets/fs117/en/
2.
Dengue [Internet]
.
South-East Asia Regional Office
.
2018
. Available from: http://www.searo.who.int/entity/vector_borne_tropical_diseases/data/data_factsheet/en/
3.
Khetarpal
N
,
Khanna
I.
Dengue Fever: Causes, Complications, and Vaccine Strategies
.
Journal of Immunology Research.
2016
;
2016
:
1
14
.
4.
Tuiskunen Bäck
A
,
Lundkvist
Å.
Dengue viruses – an overview
.
Infection Ecology & Epidemiology.
2013
;
3
(
1
):
19839
.
5.
Katiyar
and
Nath
,
In-silico Protein-Ligand Docking Studies against the NS5 Methyltransferase Protein of Dengue Virus
.
J Proteomics Bioinform
2018
,
11
:
6
6.
Bone
K
and
Mills
S
Principles and Practice of Phytotherapy Modern Herbal Medicine
2nd Edition.
2013
7.
Monica
Comalada
 et al 
In vivo quercitrin anti-inflammatory effect involves release of quercetin, which inhibits inflammation through down-regulation of the NF-B pathway
.
Eur. J. Immunol.
2005
.
35
:
584
592
8.
Babujanarthanam
R.
 et al 
Quercitrin, a bioflavonoid improves glucose homeostasis in streptozotocin-induced diabetic tissues by altering glycolytic and gluconeogenic enzymes.
Volume
24
, Issue
3
June
2010
9.
Kato
F
,
Ishida
Y
,
Oishi
S
,
Fujii
N
,
Watanabe
S
,
Vasudevan
S
 et al 
Novel antiviral activity of bromocriptine against dengue virus replication
.
Antiviral Research.
2016
;
131
:
141
147
.
10.
Li
Y
,
Yao
J
,
Han
C
,
Yang
J
,
Chaudhry
M
,
Wang
S
 et al 
Quercetin, Inflammation and Immunity
.
Nutrients.
2016
;
8
(
3
):
167
.
11.
Parasuraman
S
,
Anand David
A
,
Arulmoli
R.
Overviews of biological importance of quercetin: A bioactive flavonoid
.
Pharmacognosy Reviews.
2016
;
10
(
20
):
84
.
12.
Zheng
Y
,
Deng
G
,
Liang
Q
,
Chen
D
,
Guo
R
,
Lai
R.
Antioxidant Activity of Quercetin and Its Glucosides from Propolis: A Theoretical Study
.
Scientific Reports.
2017
;
7
(
1
).
13.
Zandi
K
,
Teoh
B
,
Sam
S
,
Wong
P
,
Mustafa
M
,
AbuBakar
S.
Antiviral activity of four types of bioflavonoid against dengue virus type-2
.
Virology Journal.
2011
;
8
(
1
):
560
.
14.
Saptawati
L
,
Febrinasari
R
,
Yudhani
R
,
Faza
A
,
Luthfiani
S
,
Ummiyati
H
 et al 
In vitro study of eight Indonesian plants extracts as anti-Dengue virus
.
Health Science Journal of Indonesia.
2017
;
8
(
1
).
15.
Lim
SP
,
Noble
CG
,
Shi
P-Y.
The dengue virus NS5 protein as a target for drug discovery
.
Antiviral Res.
2015
Jul;
119
:
57
67
.
16.
Howe
MK
,
Speer
BL
,
Hughes
PF
,
Loiselle
DR
,
Vasudevan
S
,
Haystead
TAJ
.
An inducible heat shock protein 70 small molecule inhibitor demonstrates anti-dengue virus activity, validating Hsp70 as a host antiviral target
.
Antiviral Res.
2016
Jun;
130
:
81
92
.
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