Influence of artificial light color on cellular respiration of green algae photosynthesis activity

Photosynthesis is the process by which plants use the light to produce glucose from carbon dioxide and water and then converted into pyruvate which releases adenosine triphosphate (ATP) by cellular respiration. This paper presents a biosensor using chlorella kessleri green algae as living transducer and used for cellular respiration detection in the form of producing dissolved oxygen (DO) in water. Green algae by 150 µL with colony of 2.26·10⁶ cells/ml is immobilized into biochip chamber purchased from cellasys GmbH and supplied with 50 µL Algae Culture Broth (ACB). Above the biochip, an artificial light replacing natural light is mounted to stimulate the photosynthesis mechanism of the algae, which is controlled using a computer. The influence of artificial light color for the DO production was measured using artificial light sources from LED Grow Light (GL), fluorescent tube lamp (TL) 21 watt and red or blue filtered TL lamp. The results show that the TL lamp light produces the highest DO level with an average potential of 1657.64 mV and a red filtered light with a value of 1728.77 mV. The results indicate that the TL light spectrum affects algae cell metabolism better than filtered TL light since it consists of various wavelengths needed by algae cells.