Photosynthesis is the process by which plants use the light to produce glucose from carbon dioxide and water and then converted into pyruvate which releases adenosine triphosphate (ATP) by cellular respiration. This paper presents a biosensor using chlorella kessleri green algae as living transducer and used for cellular respiration detection in the form of producing dissolved oxygen (DO) in water. Green algae by 150 µL with colony of 2.26·106 cells/ml is immobilized into biochip chamber purchased from cellasys GmbH and supplied with 50 µL Algae Culture Broth (ACB). Above the biochip, an artificial light replacing natural light is mounted to stimulate the photosynthesis mechanism of the algae, which is controlled using a computer. The influence of artificial light color for the DO production was measured using artificial light sources from LED Grow Light (GL), fluorescent tube lamp (TL) 21 watt and red or blue filtered TL lamp. The results show that the TL lamp light produces the highest DO level with an average potential of 1657.64 mV and a red filtered light with a value of 1728.77 mV. The results indicate that the TL light spectrum affects algae cell metabolism better than filtered TL light since it consists of various wavelengths needed by algae cells.

1.
R.G.
Wetzel
and
G.E.
Likens
,
Dissolved Oxygen. Limnological Analyses
, pp.
73
84
(
2013
).
2.
Y.
Zhao
,
T.
Ye
,
H.
Chen
,
D.
Huang
,
T.
Zhou
,
C.
He
and
X.
Chena
,
Luminescence
26
(
1
), pp.
29
34
(
2011
).
3.
O.K.
Agwa
,
S.N.
Ibe
, and
G.O.
Abu
,
International Research Journal of Microbiology (IRJM)
3
(
9
), pp.
288
295
(
2012
).
4.
L.A.
Lewis
and
R.
McCourt
,
American Journal of Botany
91
(
10
), pp.
1535
1556
(
2004
).
5.
W.
Blanken
,
R.H.
Wijffels
,
M.
Cuaresma
,
M.
Janssen
,
Cultivation of microalgae on artificial light comes at a cost. Algal Res.
2
, pp.
333
340
(
2013
).
6.
C.Y.
Wang
,
C.C.
Fu
,
Y.C.
Liu
,
Biochemical Engineering Journal
37
(
1
), pp.
21
25
, 15 October(
2007
),
7.
C.Y.
Chen
,
Y.C.
Chen
,
H.C.
Huang
,
C.C.
Huang
,
W.L.
Lee
,
J.S.
Chang
,
Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microalga Nannochloropsis oceanica CY2
.
Bioresour. Technol.
147
, pp.
160
167
(
2013
).
8.
C.H.
Shu
,
C.H.
Tsai
,
W.H.
Liao
,
K.Y.
Chen
,
H.C.
Huang
,
J. Chem. Technol. Biotechnol
87
, pp.
601
607
(
2012
).
9.
T.
Mooij
,
G.
Vries
,
C.
Latsos
,
R.H.
Wijffels
, and
M.
Janssen
.
Algal Research
15
, pp.
32
42
, April (
2016
).
10.
C.H.
Ra
,
C.H.
Kang
,
J.H.
Jung
,
G.T.
Jeong
and
S.K.
Kim
,
Bioresource Technology
212
, pp.
254
261
(
2016
).
11.
C.L.
Teo
,
M.
Atta
,
A.
Bukhari
,
M.
Taisir
,
A.M.
Yusuf
and
Ani
Idris
,
Bioresource Technology
162
, pp
38
44
. June (
2014
).
12.
J.
Wiest
,
T.
Stadthagen
.,
M.
Schmidhuber
,
M.
Brischwein
,
J.
Ressler
,
U.
Raeder
and
H.
Grothe
,
Analytical Letters
39
, pp.
1759
1771
(
2006
).
13.
L.
Umar
,
Y.
Hamzah
, and
R.N.
Setiadi
,
Measurement Science and Technology
30
(
6
):
65102
.(
2019
).
14.
P.
Staudacher
,
B.
Wolf
,
J.
Wiest
and
M.
Schmidhuber
.
Mobile biosensor for water quality monitoring detection of oil in biotope water with a whole-cell biosensor sistem
,
IEEE Africon 2011 - The Falls Resort and Conference Centre
,
Livingstone, Zambia
, 13 15 September (
2011
).
15.
U.
Lazuardi
,
A.A.
Frank
,
W.
Joachim
,
Application of algae-biosensor for environmental monitoring
, 2015
37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC
).
Milan Italia
(
2015
).
16.
A.P.
Carvalho
,
S.O.
Silva
,
J.M.
Baptista
,
F.X.
Malcata
,
Applied Microbiology and Biotechnology
89
, pp.
1275
1288
(
2011
).
17.
Y.
Yang
,
Effects of Temperature, Light Intensity and Quality, Carbon Dioxide, and Culture Medium Nutrients on Growth and Lipid Production of Ettlia oleoabundans
. Dissertation.
Worcester Polytechnic Institute
. (
2013
).