CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.
Skip Nav Destination
Article navigation
6 September 2019
PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations
11–12 April 2019
Bali, Indonesia
Research Article|
September 06 2019
Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene
Murdani Abdullah;
Murdani Abdullah
a)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
a)Corresponding author: murdani08@gmail.com;
Search for other works by this author on:
Budiman Bela;
Budiman Bela
b)
2
Virology and Cancer Pathobiology Research Center, Faculty of Medicine Universitas Indonesia
, Cipto Mangunkusumo National Hospital Jakarta Indonesia
Search for other works by this author on:
Ari Fahrial Syam;
Ari Fahrial Syam
c)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
Search for other works by this author on:
Marcellus Simadibrata;
Marcellus Simadibrata
d)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
Search for other works by this author on:
Sofy Meilany;
Sofy Meilany
e)
2
Virology and Cancer Pathobiology Research Center, Faculty of Medicine Universitas Indonesia
, Cipto Mangunkusumo National Hospital Jakarta Indonesia
Search for other works by this author on:
Firda Annisa;
Firda Annisa
f)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
Search for other works by this author on:
Dian Amirulloh;
Dian Amirulloh
g)
2
Virology and Cancer Pathobiology Research Center, Faculty of Medicine Universitas Indonesia
, Cipto Mangunkusumo National Hospital Jakarta Indonesia
Search for other works by this author on:
Dadang Makmun;
Dadang Makmun
h)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
Search for other works by this author on:
Abdul Aziz Rani
Abdul Aziz Rani
i)
1
Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia
Jakarta Indonesia
Search for other works by this author on:
AIP Conf. Proc. 2155, 020041 (2019)
Citation
Murdani Abdullah, Budiman Bela, Ari Fahrial Syam, Marcellus Simadibrata, Sofy Meilany, Firda Annisa, Dian Amirulloh, Dadang Makmun, Abdul Aziz Rani; Characterization of colon cancer cells from a patient at Ciptomangun Kusumo National Hospital using CD44, NANOG and OCT4 gene. AIP Conf. Proc. 6 September 2019; 2155 (1): 020041. https://doi.org/10.1063/1.5125545
Download citation file:
249
Views
Citing articles via
The effect of a balanced diet on improving the quality of life in malignant neoplasms
Yu. N. Melikova, A. S. Kuryndina, et al.
Animal intrusion detection system using Mask RCNN
C. Vijayakumaran, Dakshata, et al.
Recognition of cat ras of face and body using convolutional neural networks
Akhmad Wahyu Aji, Esmeralda Contessa Djamal, et al.
Related Content
Assessment of cluster of differentiation (CD44) as protective factor for the incidence of breast cancer
AIP Conf. Proc. (April 2023)
Mutual antagonism between CD44 and integrins in glioblastoma cell traction and migration
APL Bioeng. (July 2024)
A prognostic significance of axillary lymph nodes of cancer stem cells CD44, CD24, ALDH 1 based on pathological responses in triple negative breast cancer: A study
AIP Conf. Proc. (July 2024)
Interconnected feedback loops among ESRP1, HAS2, and CD44 regulate epithelial-mesenchymal plasticity in cancer
APL Bioeng. (August 2018)
Catch bond interaction allows cells to attach to strongly hydrated interfaces
Biointerphases (January 2016)