CD44 is a cell adhesion molecule and has been recognized as a cancer marker that plays an important role in tumor progression and metastasis. The role of CD44 in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN) and to messenger molecules, such as growth factors present in the tumor microenvironment where they contribute to the maintenance of the stemness of malignant cells. Nanog and Oct are pluripotent stem cells that play roles in development of cancer stem cell. The aim of this research was to investigate expression of CD44, Nanog and Oct 4 at colorectal patient stadium IV. Biopsy of patient was extracted using collagenese I and incubate at 37°C for 30 minutes. Upon biopsy, the tissues were analyzed using Tali cytometer and indirect immunostaining for CD44, Nanog and OCT 4 expressions at days 3, 7 and 10. The results were compared with that of colo cell line. Results revealed the significance differences in gene expression between samples from patients with line colo cells originating from Caucasian colon cancer patients. Such differences necessitated for more in-depth researches towards the gene expression of Indonesian colon cancer patients that may prove to be useful for developing future anti-cancer drugs.

1.
K.
Thanikachalam
,
G.
Khan
,
Nutrients
11
(
164
),
1
11
(
2019
).
2.
G.
William
M,
Markowitz
D
,
Dig Dis Sci
60
(
3
),
762
772
(
2015
)
3.
Langan
R
,
Mullinax
J
,
Raiji
M
,
Upham
T
,
Summers
T
,
Stoiadinovic
A
,
Avita
I
,
J. Cancer
4
,
241
250
(
2013
)
4.
Munro
M
,
Itinteang
,
Wickremesekera
,
Peng
L
,
Tan
A
,
J Clin Pathol
71
,
110
116
(
2018
)
5.
Bates
R.
,
Edwards
N
,
Burns
G
,
Fisher
D
,
Cancer Res.
61
,
5275
5283
, (
2001
)
6.
Ishiguro
T
,
Sato
A
,
Ohata
H
,
Sakai
H
,
Nakagama
,
Okamoto
,
Biochem. Biophys. Res. Commun.
48
,
199
204
, (
2012
)
7.
Zhou
,
JY
,
Oncotarget
7
(
7
),
7657
7666
, (
2016
)
8.
Abhisek
Mitra
, L. M. L.
Trends Biotechno
,
31
,
347
354
(
2014
)
10.
Jingyu
Zhang
1,
Oncogene
,
32
(
37
), p.
4397
4405
,
2013
11.
Jin-Yong
Zhou
, 2016.
Oncotarget
,
7
(
7
), pp.
7657
7666
, (
2016
)
12.
Kevin
Van der Jeught
, H.-C 2018.
World J Gastroenterol
,
34
(
24
), pp.
3834
3848
(
2018
)
13.
O’Brien
,
A. K. a. C. A
,
Current protocols in stem cell biology
(
John Wileys & Sons
,
Ontario
,
2008
)
14.
Ristides G.
Vaiopoulos
, O.
Stem Cells
,
30
, p.
363
371
(
2012
)
15.
Russell C.
Langan
, J. E.
J. Cancer
4
, pp.
241
250
(
2013
)
16.
Zhengwei
Leng
, Q. X. D,
Cell Physiol Biochem.
,
46
, pp.
860
872
(
2018
)
This content is only available via PDF.