Nanoparticle is increasing in demand especially in the area such as cosmetics, biomedical sciences, environmental health and drug delivery which mainly due to its minute particle size. Gynura procumbens (G. procumbens) is a medicinal plant that easily found in Southeast Asia and can be used to synthesize silver nanoparticles (AgNPs) without any harsh and toxic chemicals. The objectives of this study were to synthesis AgNPs using G. procumbens leaves extract and investigate the effect of concentration of silver nitrate, concentration of plant extract (PE), reaction time and ratio of PE to silver nitrate in synthesizing AgNPs. Silver nanoparticles were prepared by mixing silver nitrate with PE and the synthesized AgNPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and zeta potential analysis. It was discovered that G. procumbens leaves extract able to reduce aqueous silver ions which resulted in a colour change indicating AgNPs formation. The UV-Vis spectrum showed surface plasmon resonance peak intensity ranging from 449 to 471 nm. Zeta potential analysis proved stability of AgNPs by having the value −40 mV. The FTIR analysis showed that AgNPs were capped by various protein molecules and TEM confirmed the spherical shape of nanoparticles. The data compiled illustrated that G. procumbens is a potential natural source for synthesizing AgNPs.

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