The purpose of this experiment was to study the effect of corn starch’s transformation rate on the quality of papaya juice and discuss the its role in the fermentation process of papaya juice. Papaya grown in Lincang was taken as experimental material. During the period of 2017-2018, five repetitive experiments on gelatinization, liquefaction and saccharification of maize starch were carried out, respectively. Pure saccharified liquid, fermentable sugar used by yeast, was extracted by coarse filtration of gauze and filtration of suction filter. In 2017, the activity conditions of alpha-amylase and glucoamylase were determined. Four different saccharification methods including double enzymatic method, acid hydrolysis method, enzymatic hydrolysis method and acid enzymatic method were selected for experiments, respectively. The optimum method on saccharification of corn starch was determined according to the sugar content and yield of saccharified solution. The saccharified liquid obtained was added to peeled and non-peeled papaya juices for fermentation, respectively. During the experiment, the quality indexes, such as total sugar, total acid, tannin, anthocyanin and chroma were determined, respectively. In 2018, the transformation experiments of corn starch in corn flour, fresh corn granules and mature corn granules were meanwhile carried out. The best raw material for saccharification was selected through the total sugar content and the extraction rate of saccharification solution. The optimum conditions of enzyme activity were determined as pH 6, temperature 70 °C in α-amylase, pH 5 and temperature 60 °C in saccharifying enzyme. The yield of saccharified solution of mature corn granules was 60%, the sugar content was 193.64 g/L. The double enzymatic saccharification obtained 46 g/L sugar content and 46.60% yield of saccharified solution. Therefore, mature maize granules with double enzymes method were the best choice for saccharification experiments, which increased not only the sugar content and the yield of saccharified solution, but also made the operation simple and filter easy. The saccharified liquid extracted by mature corn granules combining with double enzymatic method could promote the fermentation of papaya wine, which, on the one hand, could protect the color of papaya wine, on the other hand, improve the flavor of papaya wine, and make papaya wine with unique honey and caramel flavor.

1.
E. C.
Ramirez
,
D. B.
Johnston
,
A. J.
McAloon
,
W.
Yee
and
V.
Singh
,
Ind. Crop. Prod.
27
,
91
97
(
2008
).
2.
A. D.
Rovira
,
Plant and Soil
7
(
2
),
178
194
(
1956
).
3.
C.
Akerberg
,
G.
Zacchi
,
N.
Torto
and
L.
Gorton
,
J. Chem. Technol. Biot.
75
,
306
314
(
2000
).
4.
J. F.
Saeman
,
J. L.
Bubl
and
E. E.
Harris
,
Ind. Eng. Chem. Analytical Edition 1
7
,
35
37
(
1945
).
5.
P. V.
Aiyer
,
Afr. J. Biotechnol.
4
(
13
), (
2005
).
6.
M.
Ceska
,
K.
Birath
and
B.
Brown
,
Clin. Chim. Acta
26
,
437
444
(
1969
).
7.
S.
Pervez
,
A.
Aman
,
S.
Iqbal
,
N. N.
Siddiqui
and
S. A. U.
Qader
,
Bmc Biotechnol.
14
,
49
(
2014
).
8.
C. C.
Parrish
, In Lipids in freshwater ecosystems (
Springer
,
New York, NY
.
1999
), pp.
4
20
.
9.
C. E.
Huckaba
and
F. G.
Keyes
,
J. Am. Chem. Soc.
70
,
1640
1644
(
1948
).
10.
J.
Lee
,
R. W.
Durst
and
R. E.
Wrolstad
,
J. Aoac Int.
88
,
1269
1278
(
2005
).
11.
J. B.
Oke
and
J. E.
Gunn
,
The Astrophys. J.
266
,
713
717
(
1983
).
12.
Y.
Nakamura
,
F.
Kobayashi
,
M.
Ohnaga
and
T.
Sawada
,
Biotechnol. Bioen.
53
,
21
25
(
1997
).
13.
T.
Baks
,
F. H.
Kappen
,
A. E.
Janssen
and
R. M.
Boom
,
J. Cereal Sci.
47
,
214
225
(
2008
).
14.
M.
Rebroš
,
M.
Rosenberg
,
Z.
Mlichová
and
M.
Paluch
,
Enzyme Microb. Tech.
39
,
800
804
(
2006
).
15.
A.
Burhan
,
U.
Nisa
,
C.
Gökhan
,
C.
Ömer
,
A.
Ashabil
and
G.
Osman
,
Process Biochem.
38
,
1397
1403
(
2003
).
16.
W.
Liu
,
W.
Huang
,
W.
Sun
,
Y.
Zhu
and
J.
Ni
,
World J. Microb. Biot.
26
:
1171
1180
(
2010
).
17.
S.
Shanavas
,
G.
Padmaja
,
S. N.
Moorthy
,
M. S.
Sajeev
and
J. T.
Sheriff
,
Biomass and Bioenergy
35
:
901
909
(
2011
).
18.
A.
Kunamneni
and
S.
Singh
,
Biochem. Eng. J.
27
,
179
190
(
2005
).
19.
L.
Li
, “
Analysis and determination of amylose content in corn starch
,” Ph.D. thesis,
Henan University of Technology
,
2018
.
20.
J. E.
Schmidt
,
T. M.
Bowles
and
A.
Gaudin
,
Front. Plant Sci.
7
,
373
(
2016
).
21.
P. V.
Van Soest
,
J. B.
Robertson
and
B. A.
Lewis
,
J. Dairy Sci.
74
,
3583
3597
(
1991
).
22.
R.
Bhosale
and
R.
Singhal
,
Carbohyd. Polym.
66
,
521
527
(
2006
).
23.
A.
Buica
,
C.
Wilson
and
J.
Brand
,
Evaluation
26
,
278
80
.
24.
Y. H.
Park
,
J.H.
Lim
and
B. J.
Seo
,
Patent Application
14
,
458
(
2014
).
25.
F.
Kobayashi
,
T.
Sawada
,
Y.
Nakamura
 et al,
Appl. Biochem. Biotech.
69
,
177
189
(
1998
).
26.
M.
Esti
,
I.
Benucci
,
C.
Lombardelli
,
K.
Liburdi
and
A. M. V.
Garzillo
,
Food Bioprod. Process.
91
,
595
598
(
2013
).
27.
X.
Wang
,
Y.
Xie
and
X.
Zhou
,
Virus Genes
29
,
303
309
(
2004
).
28.
P. R.
Lee
,
B.
Yu
,
P.
Curran
and
S. Q.
Liu
,
Food Res. Int.
44
,
1292
1298
(
2011
).