The objective is to investigate the effects of Wumei Pill on intestinal flora and neurotransmitters in rats with diarrhea-predominant irritable bowel syndrome (IBS-D). Method: The model was established by means of acetic acid enema plus restraint stress. The rat fecal moisture content, fecal trait score, and abdominal wall withdrawal reflex (AWR) score were used to determine whether the model was successful. Evaluated the Wumei Pills effect on rat fecal moisture content, fecal trait score, AWR score, Bifidobacterium/Enterobacteriaceae (B/E) value, and hippocampal tissue glutamate (Glu) and γ-aminobutyric acid (γ-GABA), dopamine (DA), 5-hydroxytryptamine (5-HT) content. Results: (1) the rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the fecal moisture content, fecal trait score, AWR score (P<0.05), and alleviate the symptoms of rat diarrhea; (3) Wumei Pills can increase the rats B/E value (P<0.05) increase the intestinal colonization resistance in rats. (4) Wumei Pills can reduce the contents of Glu, γ-GABA, DA and 5-HT in the hippocampus of rats (P<0.05), and have a certain regulatory effect on neurotransmitters in the rat brain. Conclusion: Wumei Pill has a significant therapeutic effect on IBS-D, and the mechanism may be related to the regulation of intestinal flora and neurotransmitters.

Topics
Acetic acid, Glutamate, Abdominal wall, Neurotransmitters, Neuroanatomy
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