In order to develop the new raw materials for cultivation of Flammulina velutipes and the resource utilization of rape straw, the mycelial growth, fruiting body growth and economic benefits of F. velutipes was studied in eight kinds of medium formulas (A, B, C, D, E, F, G, H) designed to cultivate F. velutipes with rape straw as the main material. Results shown that the hyphae of F. velutipes in treatment E, F and D were the strongest, and in treatment H was weak. Compared with CK, the mycelial growth rate of the eight formula media was significantly increased, and treatment E was the fastest. The average yield of treatment F was the highest, followed by treatment E. According to various indicators, the formula suitable for the growth of F. velutipes was F formula (68% rape straw, 20% cottonseed hull, 10% bran, 1% sucrose and 1% superphosphate). Cultivation of F. velutipes with rape straw can shorten the cultivation period of hyphae and the bud period and fruiting time of fruiting bodies, and increased economic and environmental benefits.

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