Growth conditions of Termitomyces albuminosus

In order to solve the problem of difficult isolation of Termitomyces albuminosus, a strain of wild T. albuminosus collected from Jiajiang County, Leshan City, Sichuan Provence,China, was isolated and purified by tissue separation method. The mycelium growth rate was used as an indicator to explore the optimal growth carbon source, nitrogen source, KH₂PO₄ concentration, MgSO₄ concentration, temperature and pH. The results showed that the optimum carbon source for vegetative mycelial growth was corn flour; the optimum nitrogen source was ammonium nitrate. The concentration of KH₂PO₄ with faster growth of T. albuminosus was between 0.10% to 0.20%, the optimum concentration was 0.15%. The concentration of MgSO₄ with faster growth of T. albuminosus was between 0.025% to 0.075%, the optimum concentration was 0.050%. The growth temperature ranges from 22 to 30°C, the optimum temperature was 26 °C. The pH range was 4.5 to 8.0, and the optimum pH was 5.0.