Sichuan Basin is one of centers of the main distribution area of pummelo (Citrus grandis Osbeck) genetic germplasms. It is necessary to analyze the genetic relationship of the local germplasms and to get enough information for protecting and utilizing them in the future. In this study, fifteen simple sequence repeat (SSR) primers mined in Citrus were used to detected the genetic diversity of 73 pummelo germplasms and rootstocks collected in Sichuan Basin. A total of 123 alleles were generated across 15 SSR loci, with an average of 8.2 alleles per locus. The observed heterozygosity (Ho) ranged from 0.0000 to 0.7429 with an average 0.2090 per locus, while expected heterozygosity (He) varied from 0.0273 to 0.6068 with an average of 0.2395 per locus. Obvious differences of genetic diversity were observed among different pummelo varieties groups. The average genetic diversity index among groups showed as follows: Na = 1.6935, H = 1.4404, I = 0.3363, Ho = 0.2570, He = 0.2470. At the group level, rootstocks of C. jonus and Poncirus trifoliata revealed the highest genetic diversity index (H = 1.9685, I = 0.6854, Ho = 0.3556, He = 0.5244), and Shatian pummelo varieties showed the lowest index (H = 1.2486, I = 0.2202, Ho = 0.1611, He = 0.1590). The highest genetic similarity coefficient (0.9668) was observed between Guanxi Miyou & bud mutants group, and Wendan pummelo varieties group, and the lowest one (0.7831) was detected between Guanxi Miyou & bud mutants group, and Putao pummelo varieties group (C. paradisi). 73 accessions of pummelo and rootstocks were clustered into five groups with UPGMA method, consisting of Shatian pummelo varieties group, Wendan pummelo varieties group, Guanxi Miyou & bud mutants group, Putao pummelo varieties group, and rootstocks of C. junos (Pujiang Xiangcheng and Ziyang Xiangcheng) and Poncirus trifoliata. No significant differentiation was observed between Guanxi Miyou and bud mutants by SSR markers.
Genetic diversity of pummelo (Citrus grandis osbeck) germplasms in sichuan Basin inferred from SSR markers
Yan Wang, Youheng Qi, Wen He, Gen Li, Tao Chen, Qing Chen, Haoru Tang, Xiaorong Wang; Genetic diversity of pummelo (Citrus grandis osbeck) germplasms in sichuan Basin inferred from SSR markers. AIP Conf. Proc. 28 February 2019; 2079 (1): 020004. https://doi.org/10.1063/1.5092382
Download citation file: