It has been reported that a phenomenon called “surgical diabetes”, which is a temporary hyperglycemic condition, occur due to anesthetic action during surgery. Surgical diabetes has been thought to be caused by the suppression of insulin secretion due to a decrease in glucose uptake. However, recent reports showed that insulin secretion is not inhibited by the low concentration of volatile anesthetic isoflurane used for treatments; though inhibition of glucose uptake occurred. Nevertheless, the cause of this has not yet been fully elucidated. In this study, we aim to verify that a change in membrane fluidity of insulin receptor (IR) causes surgical diabetes. Therefore, we have constructed a laminar flow live imaging system using a microfluidic device. The system control drug exposure within the observation system by creating a laminar flow in the cell culture place using MEMS technology. First, we formed laminar flow while observing on the stage of the confocal microscope. Furthermore, we succeeded in peeling cells which cultured in the device flow path, only in arbitrary region by trypsin treatment. Then, in order to elucidate the above phenomenon, we have investigated the effect of IR membrane diffusion was measured by the FRAP method. The result showed that the membrane fluidity of IR is enhanced during isoflurane treatment.

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