This paper investigated the determination of amygdalin content in Cassia obtusifolia L. by high performance capillary electrophoresis (HPCE) method. The borax solution of 20 mmol concentration containing 15% methanol was chosen as buffer solution. The experiment was performed at a constant voltage of 18kV and UV detection wavelength of 210 nm. The content of amygdalin in Cassia obtusifolia L. was 5.586 mg/g (RSD = 8.56%) (n = 6). The recovery was in the range of 88.2%-111.0% (n=6). This method is suitable for the detection of the content of amygdalin in Cassia obtusifolia L…

Topics
Electrophoresis, Liquid chromatography, Buffer solutions
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