Endogenous and exogenous plant growth regulators play essential role in the growth and proliferation of calluses. To establish an efficient protocol of callus proliferation, various concentrations of a-naphthaleneacetic acid (NAA) (0.4, 0.5, 0.6 mg·L−1) in combination with 6-benzylaminopurine (6-BA) (1.5, 2.0, 2.5 mg·L−1) on callus proliferation of Chinese kale were evaluated. The results showed that the best callus proliferation was observed on MS medium with 0.4 mg·L−1 NAA and 2.5 mg·L−1 6-BA after 30 d of culture, and the morphology of the callus was yellowish in colour and friable. This study provides a basis for future studies on genetic improvement for Chinese kale.
Topics
Ecology
REFERENCES
1.
Sun
B
, Liu
N
, Zhao
Y
, et al Variation of glucosinolates in three edible parts of Chinese kale (Brassica alboglabra Bailey) varieties
. Food Chem.
Vol. 124
(2011
) No. 3
, p. 941
–947
.2.
Wang
J
, Lu
Y
, Wang
Q
, et al An efficient callus proliferation protocol and rhaponticin Accumulation of Rheum franzenbachii Munt. a medicinal plant
. Journal of Plant Biochemistry & Biotechnology.
Vol. 20
(2011
), No. 2
, p. 252
–257
.3.
Murashige
T
, Skoog
F.
A revised medium for rapid growth and bioassays with tobacco tissue cultures
. Physiol Plant.
Vol. 15
(1962
), p. 473
–497
.4.
Qian
Y P
, Subramaniam
S
, Lim
V
, et al The establishment of cell suspension culture of Sabah snake grass (Clinacanthus nutans (Burm.F.) Lindau)
. In Vitro Cellular & Developmental Biology - Plant
, (2016
) No. 9
, p. 1
–10
.5.
Mun
S C
, Mun
G S.
Development of an efficient callus proliferation system for Rheum coreanum Nakai, a rare medicinal plant growing in Democratic People’s Republic of Korea
. Saudi Journal of Biological Sciences.
Vol. 23
(2016
), p. 488
–494
.6.
Kou
Y P
, Ma
G H
, Teixeira da Silva
J A
, et al Callus induction and shoot organogenesis from anther cultures of Curcuma attenuata Wall
. Plant Cell Tiss Organ Cult.
Vol. 112
(2013
) No. 1
, p. 1
–7
.7.
Gourguillon
L
, Rustenholz
C
, Lobstein
A
, et al Callus induction and establishment of cell suspension cultures of the halophyte Armeria maritima (Mill.) Willd
. Scientia Horticulturae.
Vol. 233
(2018
), p. 407
–411
.8.
Zulkarnain
, Neliyati
, Lizawati
. Callus proliferation from immature leaf explants of durian (Durio zibethinus Murr. cv. Selat) with the addition of picloram and BAP
. J. Hort. Indonesia.
Vol. 4
(2013
) No. 3
, p. 107
–114
.© 2019 Author(s).
2019
Author(s)
